大鼠Akt1基因合成及载体构建的实验研究  

Experimental Study on the Gene Synthesis and Eukaryotic Expression Vector Construction of Rattus Akt1

作　　者：吴健[1] 刘红芝[1] 张芳[1] 付燕燕[1] 王淑玲[2] 宋远见[1] 

机构地区：[1]徐州医学院,江苏徐州221004 [2]徐州医学院附属市立医院,江苏徐州221000

出　　处：《山东工业技术》2013年第12期18-18,12,共2页Journal of Shandong Industrial Technology

基　　金：徐州医学院院课题项目研究成果;项目编号2009KJ05

摘　　要：根据Genebank提供的大鼠Akt1基因序列,用PCR方法对大鼠Akt1序列进行基因合成,再将其克隆到pGCMV/MCS/RFP/Neo质粒中,而后进行NheI和BamHI双酶切法验证,为今后Akt1基因在生命科学实验中的研究打下基础。The Aktl gene was synthesized according to the gene sequences of rats from Genebank, followed by subcloning into pGCMV/MCS/ RFP/Neo expression vector. It was identified by using NheI ＋ BamHl double digestion and by sequencing. The research lays a foundation for manufacturing the protein and studying its function.

关 键 词：IAkt1基因 基因合成 载体构建 

分 类 号：Q78[生物学—分子生物学]