康复期强制戒毒人员淋巴细胞基因组DNA甲基化水平的研究  

A STUDY ON THE GENOMIC DNA METHYLATION OF LYMPHOCYTE AMONG DRUG USERS

作　　者：张凌媛[1] 康洁[1] 丁珊珊[1] 沈建英[1] 杨朝阳[1] 

机构地区：[1]福建中医药大学,福州350122

出　　处：《中国药物依赖性杂志》2013年第6期427-429,463,共4页Chinese Journal of Drug Dependence

基　　金：国家自然基金项目(30973718);国家重点基础研究发展计划(973计划)(2011CB505405);国家科技支撑计划课题(2012BAI41B01);福建中医药大学校管课题(X2011003;X2011001)

摘　　要：目的:检测康复期强制戒毒人员淋巴细胞基因组DNA甲基化的水平。方法:抽提康复期强制戒毒人员和正常人淋巴细胞基因组DNA,采用整体甲基化定量试剂盒检测基因组DNA甲基化水平。结果:分别对80例康复期强制戒毒人员和正常健康人群进行淋巴细胞基因组DNA甲基化水平检测发现,与正常组相比,康复期强制戒毒人员淋巴细胞基因组DNA甲基化百分率为15.37%±s 3.28%,显著低于正常组甲基化百分率23.18%±s 7.98%,具有统计学意义(P<0.05)。不同年龄、性别组淋巴细胞基因组DNA甲基化百分率差异不显著(P>0.05)。不同吸毒年限组中,吸毒年限为>10 a组淋巴细胞基因组DNA甲基化百分率为10.01%,显著低于其他各组,差异具有统计学意义(P=0.028)。结论:康复期强制戒毒人员淋巴细胞基因组DNA甲基化水平显著低于正常人水平,吸毒时间越长,淋巴细胞基因组DNA甲基化水平降低更为显著。Objective.：To detect methylation level of genomic DNA in lymphocytes of drug addicts under compulsory rehabilitation. Methods： Genomic DNA was extracted from lymphocytes of drug users and healthy people （control）. And DNA methylation levels were detected by Global DNA Methylation Quantification Ultra Kit. Results：Eighty drug users and 80 healthy people were involved in our study. DNA methylation level in lymphocytes of drug users was 15.37% ± s 3.28%, which was significantly lower than that of control（23.18%±s 7.98% ,P 〈0. 05）. However, there was no significant difference among different age and sexual groups （ P 〉 0. 05 ）. The DNA methylation level of drug users with drug - use history of more than 10 years was 10.01% ,which was significantly lower than that of those with less drug - use history（ P = 0. 028 ）. Conclusion ： The methylation level of genomic DNA in lymphocyte of drug users under compulsory rehabilitation was significantly lower than that of healthy people and decreased with years of drug use.

关 键 词：毒品 DNA甲基化 淋巴细胞 

分 类 号：R392-33[医药卫生—免疫学]