IL-6、IL-1β、TNFα对人胎大脑神经细胞c-fos、c-jun的表达调控  

IL-6、IL-1β and TNFα induce the expression of c-fos and c -jun in human fetal cere-bral neurons

作　　者：庞智玲[1] 李兰英[1] 魏佼[1] 尹雪茜 

机构地区：[1]天津医科大学内分泌研究所,天津300070

出　　处：《中国免疫学杂志》2000年第12期667-668,674,共3页Chinese Journal of Immunology

基　　金：天津市科委自然科学基金资助项目 !(96 310 8411)

摘　　要：目的 :探讨IL 6、IL 1β、TNFα在人胎大脑神经细胞体外发育过程中对原癌基因c fos、c jun的表达调控规律。 方法 :应用人胎大脑神经细胞无血清原代培养模型 ,通过DNA RNA斑点杂交 ,采用计算机图像分析系统测定杂交点的平均灰度值。结果 :c fos、c jun的表达均在IL 6、IL 1β、TNFα作用后 15～ 30min开始上调 ,1h达高峰 ,而后下降。 结论 :这些细胞因子均在转录水平上促进神经细胞c fos、c jun的表达 。Objective:To study the effects of IL 6、IL 1β and TNFα on the expression of c fos and c jun,which may provide the foundation for a further study in clinic.Methods:Used the a model of primary culture of human fetal cerebral neurons in serum free medium,by DNA RNA dot blot hybrdization.Results:The expression of c fos and c jun was increased between 15 min to 30 min after stimulation,reached a maximum at 1 h and declined over the subsequent 1 h.Conclusion:IL 6、IL 1β and TNF α have induce the expression of c fos and c jun during development of human fetal cerebral neurons in vitro at the level of transcription.

关 键 词：大脑神经细胞 C-FOS C-JUN IL-6 IL-1Β INFα 

分 类 号：R338[医药卫生—人体生理学] R392[医药卫生—基础医学]