黄芩苷对小鼠肝细胞色素P450的选择性诱导  

EFFECTS OF BAICALIN ON LIVER MICROSOMAL CYTOCHROME P450 SYSTEM

作　　者：侯艳宁[1] 程桂芳[1] 朱秀媛[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京 100050

出　　处：《药学学报》2000年第12期890-892,共3页Acta Pharmaceutica Sinica

摘　　要：目的　观察黄芩苷对小鼠肝细胞色素P45 0及其亚家族的影响。方法　用紫外分光光度法分别测定小鼠肝微粒体细胞色素P45 0与b5含量及氨基比林N 脱甲基酶 (ADM )、7 乙氧基香豆素O 脱乙基酶 (ECD)、苯并芘羟化酶 (AHH)活性。用蛋白印迹杂交技术鉴定细胞色素P45 0同功酶。结果　黄芩苷可使小鼠肝微粒体细胞色素P45 0含量显著增加 ,并使ADM ,ECD及AHH 3种酶活力显著增强。对 6种P45 0同功酶的鉴定结果显示 ,黄芩苷可选择性诱导 1A1,2B1及 2C113种同功酶 ,对细胞色素b5含量及 3A2 ,2D1和 2E13种同功酶无诱导作用。结论　黄芩苷对小鼠肝细胞色素P45 0有选择性诱导作用。AIM To investigate the effect of baicalin on liver microsomal cytochrome P450 system and the mechanism of liver protective action of baicalin. METHODS Liver microsomal cytochrome P450, b5, aminopyrin N demethylase (ADM), 7 ethoxycoumarin O deethylase (ECD) and benzopyrene hydroxylase (AHH) activity were quantitated by UV chromatography. Activities of six cytochrome P450 isoforms were assayed with Western Blotting. RESULTS Baicalin increased liver microsomal cytochrome P450 level and ADM, ECD and AHH activity significantly. The three P450 isoforms, 1A1, 2B1 and 2C11, were also induced selectively by baicalin, but the b5 level, 3A2, 2D1 and 2E1 were not induced. CONCLUSION Baicalin increases liver microsomal cytochrome P450 level and induces selectively 1A1, 2B1 and 2C11 of P450 isoforms in mice.

关 键 词：黄芩苷 肝细胞色素P450 药酶诱导 小鼠 

分 类 号：R285.5[医药卫生—中药学]