制首乌中1个新的四羟基二苯乙烯苷的结构鉴定及其心血管活性研究  

STRUCTURAL ELUCIDATION OF A NEW TETRAHYDROXYSTILBENE OF RADIX POLYGONI MULTIFLORI PREPARATA AND STUDY ON ITS CARDIOVASCULAR ACTIVITY

作　　者：陈万生[1] 刘文庸[1] 杨根金[1] 张卫东[1] 储智勇[1] 陈海生[1] 乔传卓 

机构地区：[1]第二军医大学药学院,上海200433

出　　处：《药学学报》2000年第12期906-908,共3页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金!( 2 9872 0 5 7);上海市科技发展基金资助项目!( 9843 19117)

摘　　要：目的　从制首乌 (RadixPolygonimultifloriPreparata)水溶性部分寻找心血管活性成分。 方法　采用体外抑制血管平滑肌细胞 (SMC)增殖活性跟踪筛选 ,利用Sephadex ,反相硅胶柱色谱、HPLC对制首乌水溶性成分进行分离纯化 ,应用理化常数测定和光谱 (UV ,IR ,MS ,1HNMR ,13 CNMR ,2D NMR)分析技术鉴定结构。结果　分离并鉴定了 1个单体化合物 2 ,3,5 ,4′ 四羟基二苯乙烯 2 O (6″ O α D 吡喃葡糖 ) β D 吡喃葡糖苷 (I)。 结论　I为新化合物 ,具有较强的抑制SMC增殖活性。AIM To investigate the cardiovascular active constituents of Radix Polygoni multiflori Preparata. METHODS Compounds were isolated from the water soluble extract with column chromatography of Sephadex, ODS and HPLC. The compounds were identified on the basis of spectral analysis (IR, EI MS, FAB MS, 1HNMR , 13 CNMR , 2D NMR) and phytochemical properties. Its inhibitory effect on the proliferation of bovine vascular smooth muscle cells were bioassayedin vitro. RESULTS One compound was isolated and identified as: 2,3,5,4′ tetrahydroxystilbene 2 O (6″ O α D glucopyranosyl) β D glucopyranoside. CONCLUSION Compound I is a new compound with cardiovascular activity.

关 键 词：制首乌 四羟基二苯乙烯苷 心血管活性 

分 类 号：R284[医药卫生—中药学]