氮离子注入柔红霉素产生菌诱变高产菌株的研究  

Study on the selection of high-producing daunorubicin strain by nitrogen ion beam injection technique

作　　者：蒋世春 白骅 陶正利 

机构地区：[1]浙江海正药业股份有限公司,台州318000

出　　处：《中国抗生素杂志》2000年第6期409-411,共3页Chinese Journal of Antibiotics

摘　　要：抗肿瘤抗生素柔红霉素产生菌 HB1482 - 170株经 1.5× 10 1 1 ions/ cm2 氮离子束诱变处理后 ,获得1株高产柔红霉素诱变株 137,菌落形态与出发株相近 ,用于生产罐 ,柔红霉素产率较出发菌株提高 2 5 .8%。A daunorubicin producing strain, Streptomyces HB 1482 170, which was initially obtained by compound mutagens such as, laser and protoplast fusion regeneration etc., was used as parent strain. It was further selected by nitrogen ion beam injection technique resulting in a mutant with stable cultural characteristics which was designated as Streptomyces No.137. The productivity of Streptomyces No.137 to produce daunorubicin in the mass prodction fermentation tanks was 25.8% higher than the parent strain.

关 键 词：氮离子注入 柔红霉素产生菌 诱变 

分 类 号：TQ465.5[化学工程—制药化工]