一种白细胞介素-2缓释微球制备新方法及体外表征  

A New Method for Interleukin-2 Release Microsphere Preparation and in Vitro Characterization

作　　者：陈顺[1,2] 赵海平[1,2] 徐健雄[1,2] 陈音[3] 郭秋圆[1,2] 黄袭雯[1,2] 陶安琪[1,2] 李晓鸣[1,2] 张向荣 林清[1,3] 

机构地区：[1]上海市第十人民医院,上海200072 [2]上海交通大学药学院,上海200240 [3]上海交通大学医学院附属新华医院,上海200092 [4]深圳赛宝儿生物药业有限公司,广东深圳518129

出　　处：《现代生物医学进展》2013年第33期6456-6459,6420,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81001416);医工交叉项目(YG2010ms11)

摘　　要：目的:开发一种白细胞介素-2(IL-2)长效缓释微球剂型。方法:采用S/O/W法制备了白介素-2因子多糖微粒的PLGA微球,考察了微球的表面形态、粒径分布等,并且运用ELISA方法考察了微球的体外释放效果。结果:本方法制备的白介素-2因子微球光滑圆整,粒径分布较均匀,体外缓释达32天,累积释放率近90%。结论:本方法制备的白介素-2因子微球,不仅具有有效地保护IL-2蛋白活性,同时实现长效缓释的目标,是一种可行的蛋白缓释方案。Objective： To develop an interleukin-2 （IL-2） long-term sustained release microsphere formulations. Methods： dextran glassy particles were prepared by a unique method of low temperature aqueous-aqueous ＂emulsion＂, and then the IL-2-1oaded dextmn glassy particles were encapsulated into PLGA microspheres by the method of solid-in-oil-in-water （S/OB/~） emulsion-evaporation technique. The microspheres were characterized by SEM and size distribution. In vitro release profiles of IL-2 loaded microspheres were plotted by Human IL-2 Quantikine ELISA Kit. Result： The IL-2-1oaded microspheres possessed spherical shape and smooth surface. The size distribution demonstrated that average spherical diameter of IL-2 microspheres was equal to 29.427 μm. The cumulative release in vitro was about 90% after 32 days. Conclusion： This method effectively protected the bioactivity of IL-2F and microspheres exhibited an ideal sustained release behavior.

关 键 词：白介素-2 缓释微球 肿瘤 

分 类 号：R94[医药卫生—药剂学] R915[医药卫生—药学]