微弧氧化法制备钛基HA/CS涂层及其生物学特性  

Titanium Based Hydroxyapatite/Chitosan Coating Prepared by Microarc Oxidation Process and Its Biological Characteristics

作　　者：王国卿[1] Zver'kov D A 张乃生[3] 

机构地区：[1]东北大学,辽宁沈阳110004 [2]莫斯科国立兽医与生物技术学院,莫斯科109472 [3]吉林大学,吉林长春130062

出　　处：《稀有金属材料与工程》2013年第12期2586-2589,共4页Rare Metal Materials and Engineering

基　　金：国家自然科学基金资助(31272615);中央高校基本科研业务费(N1104005008)

摘　　要：采用微弧氧化法在医用钛表面制备羟基磷灰石(HA)/壳聚糖(CS)涂层。通过扫描电镜(SEM)、X射线衍射(XRD)仪、红外光谱(FTIR)仪分析涂层的形貌和物相,评价HA/CS涂层对成骨细胞的毒性及其在动物体内的生物活性,分析微弧氧化法制备钛基HA/CS涂层的特征及其在骨修复中应用的可能性。结果表明:微弧氧化法可在钛表面生成HA/CS涂层,厚度40±2 m、粗糙度3.3±0.1 m、孔隙率(30±2)%,孔隙近圆形、直径几微米到20 m,结合强度20.5±1.1 MPa;涂层中HA与CS之间没有形成化学键,晶体HA占10%,平均粒径37 nm,非晶体HA占90%;钛基HA/CS涂层材料对成骨细胞增殖具有促进作用,无细胞毒性,在动物体内能够诱导骨小梁形成,具有高的生物活性。微弧氧化法制备的高生物活性钛基HA/CS涂层材料,在骨修复方面具有良好的应用前景。The hydroxyapatite（HA）/Chitosan （CS） coating was prepared by a microarc oxidation process on the surface of the biomedical used titanium. The morphology and the phase composition of the coating were analyzed by using scanning electron microscope （SEM）, X-ray diffractometer （XRD） and infrared spectrometer （FTIR）. The toxicity of the coating material to the osteoblasts and its bioactivity in vivo were evaluated. The characteristics of the HA/CS coating by the microarc oxidation method, and its possibility of application in the bone regeneration were analyzed. The results show that the HA/CS coating on the surface of titanium with a thickness of 40±2 μm, a roughness of 3.3±0.1 μm, a porosity of （30±2）%, approximately circle pores and their diameter in the range of a few micron to 20 μm, and a bond strength of 20.5±1.1 MPa, can be prepared by using the microarc oxidation method. The HA and CS in the coating do not form chemical bonds. HA crystals occupy 10% of the total particles with an average particle size 37 nm. The other 90% are amorphous HA particles. Titanium base HA/CS coating can promote the proliferation of osteoblasts and it is not toxic. In addition, it can induce the formation of bone trabecula and shows a high bioactivity. In general, the HA/CS coating material with a high bioactivity will have a good application prospect in the aspect of bone regeneration.

关 键 词：微弧氧化 羟基磷灰石 壳聚糖 复合涂层 钛种植体 生物活性 

分 类 号：R318.08[医药卫生—生物医学工程]