碳源调控下除磷/蓄磷生物滤池的生物膜特性分析  

Biofilm characteristics of an anaerobic/aerobic biofilter varied with carbon source regulation

作　　者：王凤蕊[1] 张顺[1] 田晴[1] 谢学辉[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《微生物学通报》2013年第12期2227-2236,共10页Microbiology China

基　　金：国家自然科学基金项目(No.20906011);留学回国人员科研启动基金项目(No.11W11329);上海市自然科学基金项目(No.102R1401200);中央高校东华大学基本科研业务费专项资金(No.11D11308)

摘　　要：【目的】分析厌氧/好氧交替生物滤池(AABF)在生物除磷与蓄磷过程中,采用碳源调控(定期补充进水碳源)诱导蓄磷菌群充分释磷-排磷的运行方式对滤池微生物特性的影响。【方法】通过多聚物染色、扫描电镜(SEM)以及限制性酶切多态性分析法(RFLP),对比了碳源调控前后AABF长期运行生物膜内菌群形态、组成特征及变化。【结果】AABF在实施碳源调控期间滤池生物膜内部分微生物形态主要由杆状演变为丝状;细菌种类大幅减少,碳源调控过程中生物膜内优势菌β-Proteobacteria的比例由56.9%提高至72.5%。实施碳源调控后,细菌的组成与形态变化明显。【结论】定期补充进水碳源(诱导释磷/收割磷的运行方式),同时控制碳源投量,能够引起生物膜微生物形态发生较大变化,优势菌比例迅速提高。研究结果对于通过过程调控,提高除磷工艺的设计具有重要的意义。[Objective] The effect of carbon source regulation （cyclic carbon source addition） to induce PAOs’ extensive P-release on characteristics of the microbial in a continuous anaerobic/aerobic alternating biological filter （AABF） operated for phosphorus （P） removal and accumulation was investigated. [Methods] Intracellular storage polymer dyeing, scanning electron microscopy （SEM） and restriction enzyme analysis technology of polymorphism （RFLP） were employed to characterize the changes of microbial communities in biofilm before and after the terminal carbon source regulation in the AABF’s long-term operation. [Results] During carbon source regulation period, the amount of big coccus and cocco-bacillus microbes decreased and the filamentous microbes increased instead in AABF after carbon source regulation; bacterial diversity reduced greatly, the predominance of β-proteobacteria increasing from 56.9% to 72.5%. [Conclusion] Terminal carbon source addition （induce P-release/P-recovery of operation） with corresponding dosage regulation could cause great changes in microbial morphologies and the dominate bacteria ratio increased rapidly. The results of the study provide informative reference on improving the efficiency of biological phosphorus removal system with process control.

关 键 词：生物除磷 蓄磷 生物滤池 补充碳源 强化磷释放 微生物群落 

分 类 号：X703[环境科学与工程—环境工程]