高色价栀子黄的精制工艺研究  

Preparative Separation of Crocins with High Color Value from Gardenia Fruits

作　　者：刘慧璐[1] 冯建勇[1] 王增尚 吴晨[1] 张末初 周胜[1] 刘江云[1] 郝丽莉[1] 

机构地区：[1]苏州大学药学院,江苏苏州215123

出　　处：《中国现代应用药学》2013年第12期1315-1319,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：苏州大学大学生创新创业训练计划项目(2012xj065)

摘　　要：目的对栀子黄进行进一步分离制备,以获得高色价的西红花总苷及其中主要单体成分。方法采用中压反相柱层析技术,对栀子黄进行进一步分离制备,采用液相色谱检测西红花苷-1含量,采用紫外可见光谱检测色价及其吸光度比值。结果制备工艺可有效去除绿原酸等杂质,精制后的西红花总苷样品中西红花苷-1含量为60.8%,色价高达756;并可分离获得西红花苷-1(1)、西红花苷-2(2)、西红花苷-3(3)等化合物。结论该制备方法简便、高效、成本较低,可为栀子黄的精制及产业化生产提供参考。OBJECTIVE To establish an efficient separation process to afford gardenia yellow and crocins with high color value. METHODS The process was conducted using reversed-phase medium pressure liquid chromatography. A high pressure liquid chromatography - photodiode array method was applied for qualitative and quantitative analysis of crocin-1. Moreover, the color value was also evaluated by UV method. RESULTS After the reversed-phase separation, the content of crocin-1 in refined product reached 60.8%, with the color value to be 756. Crocin-1（1）, crocin-2（2） and crocin-3（3） could be separated either with similar procedure. CONCLUSION The process developed is highly efficient, low-cost and compact, which affords a potential approach for industrial production of crocins with improved quality.

关 键 词：栀子 栀子黄 西红花苷 分离 反相柱层析 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]