UPLC同时测定百灵安神片中7种活性成分含量  

Simultaneous Determination of Seven Active Compounds in Bailing Anshen Tablets by UPLC

作　　者：赵韶华[1] 高淑丽[2] 张利康[1] 张晨光[1] 高学东[1] 刘敏彦[1] 

机构地区：[1]石家庄以岭药业股份有限公司,石家庄050035 [2]河北省石家庄市第四医院,石家庄050017

出　　处：《中国现代应用药学》2013年第12期1331-1333,共3页Chinese Journal of Modern Applied Pharmacy

基　　金："重大新药创制"科技重大专项(2011ZX09401-306)

摘　　要：目的建立UPLC同时测定百灵安神片中绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3,5-二咖啡酰奎宁酸含量的方法。方法采用Waters Acquity UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱;流动相:乙腈-0.1%磷酸水,梯度洗脱;流速:0.4 mL·min-1;检测波长:340 nm。结果绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3,5-二咖啡酰奎宁酸在检测范围内线性良好,r均>0.999 8,方法回收率分别96.3%,98.2%,100.5%,99.3%,97.7%,97.0%,96.4%。结论本方法准确可靠快捷,为全面控制百灵安神片质量提供了一种测定方法。OBJECTIVE To established a UPLC method for determination of seven active compounds in Bailing Anshen tablets, including chlorogenic acid, mangiferin, tetrahydroxystilbene, spinosin, cynaroside, quercitrin, and 3,5-dicaffeoylquinic. METHODS The chromatographic separation performed on a C18 column and with gradient elution of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.4 mL·min^-1. The detected wavelength was set at 340 nm. RESULTS The seven analytes showed good linearity （r〉0.999 8） within the test ranges. The average recoveries were 96.3%, 98.2%, 100.5%, 99.3 %, 97.7%, 97.0%, 96.4%, respectively. CONCLUSION The analytical method is simple, rapid, specific, which is useful for quality control of Bailing Anshen tablets.

关 键 词：超高效液相色谱法 百灵安神片 质量控制 

分 类 号：R917.101[医药卫生—药物分析学]