对4家猪场猪细环病毒I型的分子流行病学调查  

Molecular Epidemiological Survey of Porcine Torque Teno Virus Ⅰ in Four Pig Farms in Qingdao Area

作　　者：刘春菊[1] 孙成友[1] 赵永刚[1] 吴晓东[1] 刘文华[2] 邹艳丽[1] 任炜杰[1] 王志亮[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]青岛农业大学山东省高校预防兽医学重点试验室,山东青岛266109

出　　处：《中国动物检疫》2013年第9期68-71,共4页China Animal Health Inspection

摘　　要：为了解猪细环病毒Ⅰ型(TTV1)的流行情况,对莱西、城阳、胶州、莱阳四家规模化养猪场的59份血清进行了TTV1分子流行病学调查,从血清中提取病毒DNA,然后通过TTV1引物进行PCR检测样品的感染情况。检测结果表明,所检测的4家养猪场均检测到TTV1感染。其中,莱西某猪场的感染率最高,为55.2%;莱阳、城阳、胶州猪场的感染率分别为30%、10%、20%。目前TTV1不能造成直接的致病作用,但是在我国很容易与其他病原发生混合感染,导致协同致病,因此了解TTV1在猪场中的存在情况具有重要意义。In order to investigate the prevalence of porcine torque teno virus type Ⅰ（TTV1）,59 serum samples from four large-scale pig farms in Laixi,Laiyang,Jiaozhou and Chengyang were collected for molecular epidemiological investigation of TTV1. DNA was extracted from serum by a High Pure Viral Nucleic Acid Kit,and PCR was conducted using the specific primers of TTVI. Results showed that the infection of TTV1 was detected in all the four selected pig farms. Among the four farms,the highest infection rate was 55.2% in Laixi. The infection rates of farms in Laiyang,Jiaozhou,and Chengyang were 30%,10%,and 20% respectively,indicating that TTV1 existed widely in pig farms. TTV1 usually is not a pathogenic agent,but it can enhance the pathogenicity of the other pathogens.Therefore,it is important to be aware of the infection of TTV1 in pig population.

关 键 词：猪 细环病毒Ⅰ型 PCR 琼脂糖凝胶电泳 分子流行病学调查 

分 类 号：S858.28[农业科学—临床兽医学]