火炮打击兔应激防护模型心脏病理及p38表达的形态学研究  

Morphological study of pathology and p38 on Heart injury in stress protective model of rabbits struck by mountain regiment attacking artillery

作　　者：林村河[1,2] 刘胜利[1,2] 郁毅刚[1,2] 张建华 周龙[1,2] 林进皇 

机构地区：[1]解放军第一七五医院 [2]厦门大学附属东南医院神经外科 [3]解放军73131部队

出　　处：《临床和实验医学杂志》2013年第23期1873-1877,共5页Journal of Clinical and Experimental Medicine

基　　金：全军十一五医学科研基金面上项目(06MB152);福建省漳州市科技计划项目(Z10071);南京军区医学科技计划项目(12MA065)

摘　　要：目的探讨实弹演习火炮打击后,不同位置掩体保护下兔心脏损伤病理变化及丝裂原活化蛋白激酶p38(p38-MAPK)表达。方法闽南山地团进攻演习,设置远离演习场的空白对照实验兔组,火炮打击区域设置炮火覆盖山体反角近距隐蔽组和迎面角近距隐蔽组。火炮打击应激损伤后6 h,各组存活兔取心脏,HE染色观察其病理损伤形态学,观察改进型SP法免疫组化染色检测心脏p38-MAPK表达。结果与空白对照组比较,反角隐蔽组和迎面角隐蔽组p38-MAPK表达强阳性,迎面角组心脏结构紊乱,细胞数减少,基质间充血,血管痉挛狭窄,微血栓形成。p38-MARK免疫反应强度亦高于反角隐蔽组。结论火炮打击后实验兔应激损伤反应强烈,反角掩体保护减轻心脏损伤,p38-MAPK参与心脏损伤病理过程。Objective The pathology and p38 of rabbits hidden in different position of blind age were studied after the stress of attacking artillery in battlefield. Methods During the autumn regiment attack exercise in South Fujian hilly area, all experimental rabbits were divided into 3 groups （blank group, reverse hidden group and frontage hidden group）according to their concealed location. Six hours after stress stimulation of artillery, all the heart of the surviving rabbits were taken out and analyzed. Heart tissue was investigated to study positive expression of p38 after SP and HE stained. Results The hidden groups had a higher expression of p38 comparing with the control group. The frontage hidden group had a higher expression of p38 comparing with reverse hidden group. Conclusion The stress response in all experimental rabbits after striking of artiller- y is very strong. However, different position of blind age can significantly influence their survival and the degree of heart injury. Reverse hidden can relieve heart injury.

关 键 词：兔 火炮打击 应激防护模型 心脏 P38 

分 类 号：R82[医药卫生—临床医学]