MAPK特异性抑制剂SB203580对弥漫性脑创伤大鼠海马区Homerla表达及学习记忆的影响  

Effects of SB203580 on Homerla esprtssion in hippocampus and learnirig-memory after diffuse brain inju-ry in rats

作　　者：赵雅宁[1] 景丽伟[1] 张盼[1] 陈长香[1] 李淑杏[1] 李建民[2] 

机构地区：[1]河北联合大学康复医学院,唐山063000 [2]河北联合大学康复医学院附属医院,唐山063000

出　　处：《中华行为医学与脑科学杂志》2013年第12期1067-1069,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：河北省科技厅吏撑课题（2027610219）;河北省教育厅重点课题（ZD2010106）

摘　　要：目的探讨SK03380对弥漫性脑创伤大鼠海马区nmrmla表达及学习记忆的影响。方法 雄性Sprague-DawUey大鼠分为对照组、弥漫性脑创伤（diffuseh.ainiiy，DBI）组和DBI ＋ SB203580干预 组（腹腔注射，0.01应用电镜观察海马区神经细胞形态变化；免疫组化法检测Homerla和磷酸化P38MAPK表达水平；水迷宫测试动物学习记忆功能。结果与对照组比较，DBI组大鼠海马区神经细胞及 突触损伤明显，Hotwrla蛋白表达增加（6.2.96上12.74,1.28±0.10，P＜0.05）,磷酸化P38MAPK表达增加 （76.98± 16.64,2.28J：0.40. P＜0.05），动物潸伏期延长[（7＜1.64± 8.96） s, （2,4.96＋ 4.98） s, P＜0.05]，穿台次数 减少（4.48±U2,12.65±2.36. P＜0.05）,与DBI组比较，DBI＋SB203580组中大鼠海马K神经细胞及突触损 伤程度减轻，Homerla表达显著增加（79.82± 16.64，02.96土 12.74，P＜0.05）,磷酸化P38MAPK表达降低 （54.82±12.48,76.98±16,64, P＜0.05）,动物潜伏期缩短[（46.72±6.58）s, （74.64±8.96）s,p＜0.05]，穿越平台 次数增加（7.56±1.20,4.48：t1.12，p＜0-05）结论SB203580可促进DBI大鼠学习记忆功能恢复,其机制与 抑制P38MAPK磷酸化提高Homerla表达有关。OhjecUve To explore the effects of SB203580（ a MAPK inhibitor） on the expression of Ho-merla in hippocampus and learning-memory after diffuse brain injury in rats. Method Male Sprague-Dawlley rats were divided randomly inlo three groups ： control group,diffuse brain injury（ DBI） group and DBI＋ SB203580 group （peritoneal injection ,0.01 ｜xp/kg） .Mo^pliological changes of neuronaJ cells were observed by electron microscope and the expression of Homer 1 a and pboyphoiyiated p38MAPK was detected by immunohistochemistry and learning and memory functions were pctforrned with Morris water maze （ MWM）. Results Compared with control group, ultrastructure of neuronal cells and synapses were significantly.The levels of phosphorylated p38MAPK（76.98±16.64,2.28＋0.40, P〈0.05） and Homerla （62.96± 12.74,1.28±0.10, P〈0.05）respectively were increased after injury impaired.MWM test showed ll＇.at the escaping latency was prolonged（ （74.64± 8.96） s,（ 24.96±4.98） s,P〈0.05） and the frequency of crossing the platform was decreased（4,48±1.12,12,.65±2.36,P〈0.05） . Compared with the model group,SB203580 decreased ultrastructure impariment in neuronal cells and synapses and decreased phos-phorylated p38MAPK expression（54.82± 12.48,76.98±16.64, P〈0.05） and increased Homerla expression（54.82 ±12,48,76.98±16.64, P〈0.05） . MWM test showed that the escaping latency was shorten （ （46.72±6.58） s, （ 74.64±8.96）s,p〈0.05） ,and the frequency of crossing the platform was increased（7.56±1.20,4.48±1.12, Ac0.05）.Conclusion SB203580 improves th^ leaniing-memory recovery after DBI, which is related to inhibition of p38MAPK activation and increasing Homer I a expression.

关 键 词：弥漫性脑创伤 Homer1a 丝裂原活化蛋白激酶 记忆 

分 类 号：R651[医药卫生—外科学]