甘肃不同产地黄芪与红芪的ISSR指纹图谱鉴别研究  

ISSR Analysis on Radix Astragali and Radix Hedysari from different regions of Gansu

作　　者：张蔓 孙红国[1] 吴海燕 宋志学[1] 纪鹏[1] 华永丽[1] 魏彦明[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省临夏回族自治州卫生学校,甘肃临夏730400

出　　处：《时珍国医国药》2013年第12期2917-2919,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31272600);甘肃省科技支撑计划-社会发展类(No.1204FKCA161)

摘　　要：目的 对甘肃不同产地黄芪、红芪共27个居群的样品进行基因组多态性分析.方法 采用ISSR分析标记方法,从120个ISSR引物中筛选出3个多态性引物分别对黄芪、红芪样品进行DNA扩增.结果 经聚丙烯酰胺凝胶电泳检测到黄、红芪共扩增出位点数23个,多态性位点数16个,平均多态性比率71.3％.利用UPGMA法构建的聚类分析表明黄、红芪样品遗传距离在0.49 ～0.88,平均为0.530 2.结论 所选3个引物可作为高特异性引物,可根据聚丙烯酰胺凝胶上显示的带型差异准确鉴别黄芪和红芪,该法具有准确、快速的特点.Objective Radix Astragali and Radix Hedysari of 27 population from different regions of Gansu was analyzed by DNA amplification. Methods Three ISSR primers selected from 120 primers tested were detected by ISSR technique. Results Three IS- SR primers were amplified a total of 23 bands by polyacrylamide gel electrophoresis ,among which 16 were polymorphic and poly- morphic loci ratio was 71.3 %. Astragalus and Raddix hedysari genetic distance in 0.49 ~ 0.88, which was an average of 0.5302, were detected by UPGMA clustering analysisConclusion Three primers can be used as high specific primer. Radix Astragali and Radix Hedysari were correctly identified by polyacrylamide gel was displayed on the belt type difference. This method was accurate and fast.

关 键 词：黄芪 红芪 ISSR 

分 类 号：R282.5[医药卫生—中药学]