肺炎衣原体感染对小鼠内皮功能及动脉粥样硬化发展的影响  

Impact of Chlamydia pneumoniae infection on endothelial function and development of atherosclerosis in mice

作　　者：李永强[1] 董吁钢[2] 马虹[2] 王丽霞[1] 王山岭[1] 段红艳[1] 

机构地区：[1]河南省人民医院心内科,郑州市450003 [2]中山大学附属第一医院心内科

出　　处：《中国临床研究》2013年第12期1292-1295,F0002,共5页Chinese Journal of Clinical Research

摘　　要：目的研究肺炎衣原体(CP)感染对小鼠内皮功能及动脉粥样硬化形成的影响及其机制。方法48只C57BL/6J小鼠随机分为感染-高脂组、高脂组、感染组和对照组(每组12只),喂养40周,作血清抗CP抗体、血脂水平及血浆一氧化氮(NO)浓度检测,取主动脉根部标本分析动脉粥样硬化斑块面积,主动脉弓部标本检测一氧化氮合酶(NOS)活力。结果感染-高脂组、高脂组和感染组小鼠血浆NO水平分别为(83.04±38.35)、(57.67±26.71)、(46.59±24.49)μmol/L,均明显高于对照组(38.56±24.97)μmol/L(P均﹤0.01)。感染-高脂组、高脂组、感染组和对照组的主动脉组织总NOS活力分别为(4.28±2.41)、(5.46±3.30)、(7.51±3.64)、(8.81±4.04)U/mg,感染-高脂组总NOS活力较对照组明显减低(P<0.05),高脂组、感染组及对照组总NOS活力比较无统计学差异(P均>0.05);感染-高脂组、高脂组和感染组小鼠主动脉组织诱生型NOS(iNOS)活力分别为(0.649±0.217)、(0.443±0.193)、(0.516±0.208)U/mg,组间水平相近(P>0.05),对照组未检测到iNOS活力。感染-高脂组小鼠平均粥样硬化斑块面积较高脂组增大[(135 249±43 748)μm2vs(96 378±30 945)μm2,P<0.05]。结论 CP感染可加速高脂饮食小鼠的主动脉粥样硬化发展,并引起主动脉iNOS产生增多,提示内皮功能异常可能是CP感染加速动脉粥样硬化发展的机制之一。Objective To investigate the influence of Chlamydia pneumoniae （CP） infection on endothelial function and forming/development of atherosclerosis and its potential mechanism in mice. Methods Forty-eight male C57BL/6J mice were randomly divided into four groups （n = 12 each） ： infection-high fat diet group （inocula- ting CP and high fat forage feeding）, high fat diet group （inoculating PBS and high fat forage feeding）, infection group （inoculating CP and ordinary forage feeding） and control group （inoculating PBS and ordinary forage feed- ing）. After 40 weeks, venous blood sample was taken and the mice were killed. Serum CP antibody, blood lipid levels and plasma nitric oxide （NO） concentration were detected. Atherosclerosis plaque area in aortic root sample was analyzed, and NO synthase （NOS） activity in aortic arch sample was determined. Results The plasma NO lev- els in infection-high fat diet group, high fat diet group and infection group were （83.04 ± 38. 35）, （57.67 ± 26. 71 ） and （46.59 ± 24. 49）ttmol/L, respectively, and their levels were all higher than that in control group （38.56 ± 2± 97 ） μmol/L （ all P 〈 0.01 ）. The activities of total NOS of aortic tissues in infection-high fat diet group, high fat diet group, infection group and control group were （±28 ± 2.41 ）, （5.46 4- 3.30）, （7.51 4- 3.64）, （8. 81 4-±04） U/mg, respectively, in which the total NOS activity in infection-high fat diet group signifi- cantly decreased compared with control group （ P 〈 0.05 ）, while there were no significant difference among high fat diet group, infection group and control group （all P 〉 0.05 ）. The inducible NOS （iNOS） activities of aortic tissues in infection-high fat diet group, high fat diet group and infection group were （0. 649 ±0. 217）, （0.443 ±0. 193） and （0.516 ± 0.208 ） U/mg, respectively, and there were no significant differences in these 3 groups （P 〉 0.05 ）, while iNOS activity in control gr

关 键 词：肺炎衣原体 动脉粥样硬化 一氧化氮合酶 诱生型 内皮功能 

分 类 号：R543.5[医药卫生—心血管疾病]