鸡Bcl-2 SYBR GREEN实时荧光定量PCR检测方法的建立与应用  

Establishment and Application of SYBR GREENⅠ Real-time Fluorescent Quantitation PCR for Detection of Bcl-2 in Chicken

作　　者：李岩[1] 刘畅[1] 刘超男[1] 高雪丽[1] 吕晓萍[1] 胡丽君[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国家禽》2013年第23期16-20,共5页China Poultry

基　　金：国家自然科学基金项目(30972162)

摘　　要：Bcl-2是与细胞凋亡相关因素的重要成员之一,是目前研究细胞凋亡的热点。本研究根据GenBank中已发表的鸡β-actin和Bcl-2基因序列设计引物,成功建立了检测鸡Bcl-2 mRNA表达的荧光定量PCR方法,并成功应用于检测益生菌对传染性法氏囊病(IBD)疫苗免疫后的雏鸡免疫器官中Bcl-2 mRNA表达量的变化,结果显示益生菌联合IBD疫苗免疫雏鸡后,其免疫器官中Bcl-2 mRNA表达量高于IBD疫苗单独免疫组。此方法具有快速、高通量、线性范围广、特异性强、灵敏度高等特点,为进一步定量研究鸡Bcl-2 mRNA表达及其与相关疾病的关系奠定了技术基础。Bcl-2 is an important member of the apoptosis-related factors,which has been a hot topic of apoptosis research currently. According to the chicken β-actin gene and Bcl-2 gene sequences published in GenBank,fluorescent quantitation PCR for detecting the expression of Bcl-2 mRNA in chicken was successfully established, and which was applied to detecting the expression of Bcl-2 mRNA in immune organs after immunized with probiotics and infectious bursal disease （IBD vaccine）, The results showed that the expression of Bcl-2 mRNA in immune organs was higher than IBD vaccine immunization alone group. This method was characterized by rapidity, high-flux, wide linear range, good specificity and high sensitivity. So this method would provide the basis for making further efforts for quantitative research on the expression of Bcl-2 mRNA and laying technical foundation for the related diseases study.

关 键 词：鸡 BCL-2基因 实时定量PCR 

分 类 号：S858.23[农业科学—临床兽医学]