柴胡皂苷d对SH-SY5Y细胞ERK蛋白表达及凋亡的影响  

Effects of Saikosaponin d on ERK1/2 Expression and Cell Apoptosis in SH-SY5Y Cells

作　　者：李妍[1] 纪朋艳[2] 张巍[1] 彭顺利[3] 吕士杰 

机构地区：[1]吉林医药学院生物化学与分子生物学教研室,吉林吉林132013 [2]吉林医药学院生物化学与分子生物学实验室,吉林吉林132013 [3]吉林医药学院临床医学院,吉林吉林132013

出　　处：《中国医科大学学报》2013年第12期1091-1094,共4页Journal of China Medical University

基　　金：吉林省科技厅科研项目(201205079);吉林市科技局科研项目(201162511)

摘　　要：目的初步研究不同浓度的柴胡皂苷d（SSd）对SH—SY5Y细胞ERK蛋白磷酸化及细胞凋亡水平的影响。方法体外常规培养人神经母细胞瘤SH-SY5Y细胞，将SSd溶解于含0．3％DMSO的DEAM高糖培养基q-，分别以0～10μmol／LSSd作用于SH—SY5Y细胞48h，之后用Westernblot法检测不同浓度的SSd对SH—SY5Y细胞t-ERK1／2及p-ERK1／2蛋白表达量的影响，并通过Hoechst33342染色及流式细胞仪检测细胞凋亡水平的变化。结果与对照组相比，8～10μmol／LSSd作用48h，SH—SY5Y细胞中ERKl／2蛋白磷酸化水平明显降低；而与对照组相比，8—10btmol／LSSd预处理的SH—SY5Y细胞凋亡细胞数明显增加，且随着SSd浓度的增加，凋亡细胞数也相应增多。结论一定浓度的SSd可引起SH—SY5Y细胞凋亡率的上升，其机制可能与抑制ERK蛋白磷酸化表达有关。Objective To investigate the effects of Saikosaponin d （SSd） on p-ERK1/2 expression and apoptosis in human neuroblastoma cells （ SH-SYSY）. Methods SH-SYSY cell lines were established in vitro firstly, then SSd was dissolved in high glucose DMEM cultures which includ- ing 0.3% DMSO. The cultures were individually pretreated with 0-10μmol/L SSd for 48 hours, then the expressions of t-ERKI/2 and p-ERK1/2 were analyzed by western-blotting. Meanwhile, cell apoptosis levels were analyzed by Hoechst33342 and flow cytometry. Results A significant re- duciton of p-ERK1/2 was observed in SH-SYSY cells after 48 h treatment of SSd at concentrations between 8-10 μmol/L. It was also shown that SSd from 8-10 μmol/L groups could induced cell apoptosis of SH-SY5Y cells. Conclusion These results demonstrate that SSd can promote the apopto- sis of SH-SYSY cells, and the mechanism may be related to the inhibition of ERK1/2 active expression.

关 键 词：ERK1 2 凋亡 柴胡皂苷D SH—SY5Y细胞 

分 类 号：R963[医药卫生—微生物与生化药学]