流式细胞术计数CD34阳性细胞的标准化与质量控制  

Standardization and Quality Control in Flow Cytometric Enumeration of CD34^+ cells

作　　者：刘艳荣[1] 陈珊珊[1] 于弘[1] 

机构地区：[1]北京大学血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2000年第4期302-306,共5页Journal of Experimental Hematology

摘　　要：用流式细胞术计数脐带血 ,外周血及其采集物中的CD34+ 细胞数已被普遍采用。然而 ,不同实验之间的CD34+ 细胞计数差异非常大。本文就影响结果的主要因素 ,目前国际上采用的标准化方案和不同方案之间的比较进行综述。我们实验室应用ProCOUNT试剂盒检测了 4 5例脐带血 ,12份正常骨髓和 4例外周血采集物中的CD34+ 细胞的数量 ,结果表明ProCOUNT为标准化程度较高的方法 ,有利于减少不同实验之间CD34+The enumeration of CD34 + cells by flow cytometry is commonly employed to assess hematopoietic stem/progenitor cells in cord blood, peripheral blood and apheresis products. Interlaboratory variation of CD34 + cells enumeration is exceedingly large. Factors contributing to those variation, currently established flow cytometry protocols and comparison between these protocols were reviewed. CD34 + cells in 45 cord blood samples, 12 normal bone marrows and 4 apheresis products were also enumerated in our laboratory by using ProCUNT kit, and results showed that the ProCUNT is highly standardizaed and could assist in reducing interlaboratory variation of CD34 + cells.

关 键 词：CD34细胞 流式细胞术 标准化 质量控制 

分 类 号：R457.9[医药卫生—治疗学]