利用光谱方法分析木腐菌降解机制的研究进展  

Research Progress on the Degradation Mechanism of Wood-rot Fungi with Spectral Analysis Methods

作　　者：吴雪君[1] 司静[1] 周金池[2] 

机构地区：[1]北京林业大学林学院微生物研究所,北京100083 [2]北京林业大学分析测试实验中心,北京100083

出　　处：《生物技术通报》2013年第12期49-55,共7页Biotechnology Bulletin

摘　　要：利用木腐菌对木质纤维素及芳香族类化合物进行生物降解具有环保、高效、低耗能等优点,而研究其降解机制对于开发生物质能源、保护生态环境等方面具有重要意义。近年来,核磁共振、红外及紫外光谱学方法已在该领域有广泛应用。基于以上,综述这三种光谱分析方法应用于探索木腐菌降解机制方面的研究进展,指出核磁共振光谱主要应用于木质结构鉴定、腐朽方式分析等方面;红外光谱可对降解机制进行深入探讨;紫外光谱方法则能够监测降解的顺序。同时还提出现阶段存在的问题,并对未来进行展望,旨在为更好地利用木腐菌进行生物降解提供相应的理论依据。Biodegradation is definitely an attractive tool which is currently of interest, as this is an environment-friendly and cost-competitive alternative. To date, wood decaying fungi are found to be among the most potent higher microorganisms in depolymerizing lignocellulose and degrade recalcitrant aromatic compounds. Exploring the mechanism of biodegradation by wood decaying fungi is an important job in the area of developing biomass energy and protecting ecological environment. Until now, nuclear magnetic resonance spectroscopy（NMR）, infrared spectroscopy（IR）and ultraviolet spectroscopy（UV）have been employed for investigating the biodegraded mechanism. The purpose of the present study is to summarize the progresses of studying the biodegraded mechanism of wood decaying fungi using the three spectroscopy analytical methods, pointing that NMR can be applied for woody structure identification and decayed pattern analysis, IR can make an in-depth exploration for biodegraded mechanism, and UV can monitor the biodegraded order. Additionally, current problems on application of the spectroscopy analytical methods in biodegradation and the future work were also proposed, which may provide the theoretical basis for making better use of wood decaying fungi in biodegradation.

关 键 词：光谱分析 木腐菌 降解能力 

分 类 号：S781.52[农业科学—木材科学与技术]