RACE法克隆亚麻COMT基因及生物信息学分析  

RACE Cloning and Bioinformatics Analysis of Flax COMT Gene

作　　者：龙松华[1] 乔瑞清[1] 陈信波[2] 邱财生[1] 邓欣[1] 郭媛[1] 郝冬梅[1] 王玉富[1] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205 [2]湖南农业大学作物基因工程湖南省重点实验室,长沙410128

出　　处：《生物技术通报》2013年第12期78-83,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31000745);国家麻类产业技术体系(CARS-19-E14)

摘　　要：利用RACE技术克隆得到亚麻木质素合成关键酶基因COMT基因的cDNA全长序列,该基因cDNA全长1 726 bp(GenBank登录号为KC832865)。含有1 104 bp完整的开放阅读框,5'非编码区长423 bp,3'非编码区长199 bp。对序列进行生物信息学分析的结果 :编码的蛋白由367个氨基酸组成(其中亮氨酸38个,丙氨酸32个和丝氨酸28个),相对分子量为40 kD,等电点pI为5.7;蛋白质二级结构以无规则卷曲和α-螺旋为主;构建系统进化树表明与赤桉(Eucalyptus camaldulensis)亲缘关系最近。Full length of gene of flax COMT was cloned using the method of RACE. The gene was 1726 bp in length, of which, the open reading frame was 1 104 bp, encoding 367 amino acid residues and having a molecular weight of 40 kD, with a 5＆apos; non-coding region of 423 bp and a 3＆apos; non-coding region of 199 bp. The gene was submitted to GenBank with a number of KC8328645. The coding protein was with pI of 5.7, in secondary structure of protein of which, random coil and α-helix were abundant category. Phylogenetic tree based on COMT genes submitted to GenBank of different plant showed that flax clustered together with Eucalyptus camaldulensis.

关 键 词：亚麻 木质素 COMT基因 RACE 生物信息学分析 

分 类 号：S563.2[农业科学—作物学]