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作 者:薛仰全[1,2] 余四九[1] 孙俊峰[2] 倪兴军[2]
机构地区:[1]甘肃农业大学动物医学院,兰州730070 [2]酒泉职业技术学院,酒泉735000
出 处:《生物技术通报》2013年第12期94-98,共5页Biotechnology Bulletin
摘 要:通过在酒泉地区应用推广超数排卵技术,探讨当地供体母牛的选择基本条件及最佳超排季节。采用自然发情+FSH+PGF2α法和CUE-MAT孕酮栓+FSH+PGF2α法研究了不同激素处理、季节、供体母牛体况、胎次等因素对超数排卵效果的影响。结果表明,自然发情法和CUE-MATE法超排的平均可用胚胎数分别为4.54枚和4.86枚,上、中、下3等膘情获得的平均可用胚胎数分别为6.13枚、5.39枚、3.29枚,春季平均可用胚数(4.00枚)<秋季平均可用胚数(4.86枚)<冬季平均可用胚数(5.07枚),经产母牛和青年母牛平均可用胚数分别为9.06枚和9.65枚。由此可见,酒泉地区供体母牛可选取膘情体况在中等以上,2-6胎经产母牛或1.5-2.0岁未产青年母牛为宜,时间在秋、冬两季节进行超数排卵较为理想。The basic conditions for selecting the local cow donors and the best seasons of their superovulation were evaluated through applying and spreading the superovulation technologies in Jiuquan area of Gansu province. The superovulation effects was examined under different factors of hormone treatments, seasons, body conditions of the cow donors and births by the methods of spontaneous estrus+FSH+PGF2α and CUE-MAT progesterone suppository+FSH+PGF2α. The results showed that the average embryos available ovulated by spontaneous estrus and CUE-MATE were 4.54 and 4.86, respectively;the average embryos available obtained by upper, middle and lower fatness were 6.13, 5.39 and 3.29, respectively;the average embryos(4.86)available in autumn was greater than that(4.00)in spring, but less than that(5.07)in winter;and the average embryos available by the multiparous cows and virgin heifers were 9.06 and 9.65, respectively. As for the cow donors in Jiuquan area, it was suitable to select multiparous cows of 2 to 6 births or virgin heifers of 1.5 to 2.0 years old, body fatness above the average, and both autumn and winter were appropriate seasons for superovulation.
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