响应面法优化降解有机氮细菌N24的种子培养基  

Optimization of Seed Culture Medium of Aerobic Denitrification Bacteria Bacillus flexus N24 Through Response Surface Methodology

作　　者：郭端强[1] 李轶徽 陈艳艳[1] 单林娜[1] 

机构地区：[1]河南城建学院生命科学与工程学院,平顶山467036 [2]平顶山市第一高级中学数学教研组,平顶山467000

出　　处：《生物技术通报》2013年第12期167-172,共6页Biotechnology Bulletin

基　　金：河南省科技攻关重点项目(092102310069)

摘　　要：优化降解有机氮细菌N24的种子培养基来提高发酵液对数期末期的细菌浓度。首先用Plackett-Burman设计对影响降解有机氮细菌N24菌体浓度的因素进行评估并筛选出具有显著效应的3个因素蛋白胨、K2HPO4和FeSO4·7H2O,接着用最陡爬坡试验法逼近以上3个因素的最大响应区域后,采用Box-Behnken设计以及响应面分析法确定3个因素的最优水平。优化后的种子培养基:蛋白胨6.55 g,K2HPO40.66 g,FeSO4·7H2O 0.024 g,NaC1 0.3g,MgSO4·7H2O 0.6 g,蒸馏水1 000 mL,初始pH值7.2。优化后发酵液对数期末期细菌浓度达到2.4440×1010CFU/mL,比优化前6.2467×109CFU/mL提高了2.91倍。Plackett-Burman设计和响应面相结合的试验方法优化了菌株N24的种子培养基,可大大提高菌株N24的细菌浓度,有望用于大规模生产。The seed culture medium was optimized to improve the cell density of strain N24. Firstly, Plackett-Burman design was used to investigate the effects of different factors on cell density in the seed culture medium, three statistically significant factors are：peptone, K4HPO2 and FeSO4 ？7H2O, and then a steepest ascent procedure were employed to define optimal response region for these three factors. Finally, Box-Behnken design and RSM were employed to define the optimal level of these three factors. The optimal seed culture medium is：peptone 6.55 g, K2HPO4 0.66 g, FeSO24·7H2 2O 0.024 g, NaC1 0.3g, MgSO 4·7H2O 0.6 g, distilled water 1 000 mL, initial pH value 7.2. The bacteria concentration reached 2.4440×1010 CFU/mL at the telophase of logarithmic phase after optimization, which increased 291% than before. The cell density of strain N24 can be increased by the optimized seed culture medium through Plackett-Burman design combined with response surface analysis, which can be used industrially.

关 键 词：降解有机氮细菌 响应面法 优化 种子培养基 

分 类 号：Q93[生物学—微生物学]