基因枪法转化大豆未成熟子叶影响因素的研究  

The Impact Factors of Immature Cotyledons Transformation via Particle Bombardment in Soybean

作　　者：王萍[1] 李倩[1] 王罡[2] 冯远航[1,2] 季静[2] 王恩旭[3] 邱丽娟[4] 

机构地区：[1]淮海工学院海洋学院 [2]天津大学环境科学与工程学院 [3]中国人民解放军92323部队农副业基地 [4]中国农业科学院作物科学研究所

出　　处：《大豆科学》2013年第6期735-739,共5页Soybean Science

基　　金：国家转基因生物新品种培育重大专项(2011ZX08004-001);中央财政支持地方高校发展专项资金(CXTD07)

摘　　要：以大豆未成熟子叶为外植体,采用基因枪轰击法进行遗传转化,探讨大豆基因型、受体轰击前预培养时间、轰击次数、轰击距离等因子对大豆未成熟子叶基因枪转化的影响。结果表明:大豆基因型对未成熟子叶基因枪转化有较大的影响,在参试的22个基因型中有40.91%的基因型体细胞胚胎发生率大于40%,胚数在2个以上,适宜用作大豆遗传转化的受体;合丰25未成熟子叶在轰击前预培养10 d优于预培养5和14 d。基因枪轰击1或2次对转化没有影响。基因枪轰击距离对转化的影响依大豆基因型而异。Using immature cotyledons of soybean as explants, the effects of soybean genotype, pre-culture time of receptor before bombardment, bombardment times and target distance on transformation immature cotyledons of soybean via particle bombardment were investigated. The results showed that genotype of soybean had obvious effects on transformation of immature cotyledon. Rates of somatic embryogenesis in 40.91% of 22 genotypes used in the experiment reached more than 40% with at least 2 embryo. These genotypes were fitted as receptor for further transformation. Before Hefeng 25 was transformed by particle bombardment, treatment with 10 days pre-cuhured was superior to those with 5 and 14 days pre-cuhured treatment. Bombardment times did not affect on transformation. Effect of target distance on transformation varied with soybean genotypes.

关 键 词：大豆 未成熟子叶 体细胞胚 基因枪法 遗传转化 

分 类 号：S565.1[农业科学—作物学]