5份春大豆苗期耐低温性的鉴定及评价  

Identification and Evaluation of Seedling Cold Tolerance of Five Spring Soybean Germplasms

作　　者：田鑫[1] 何小玲[1] 顾卫红[2] 麻浩[1] 

机构地区：[1]南京农业大学农学院/作物遗传与种质创新国家重点实验室,江苏南京210095 [2]上海市农业科学院园艺研究所,上海201106

出　　处：《大豆科学》2013年第6期755-762,共8页Soybean Science

基　　金：国家自然科学基金(30971840;31171572);高等学校博士学科点专项科研基金(20100097110030;20120097110025);上海市科委重点攻关项目(12391900900);江苏省高校优势学科建设工程资助项目

摘　　要：低温胁迫严重影响春大豆发芽、幼苗生长和发育。为建立耐低温种质的筛选方法,以5份春大豆种质为材料,测定和评价了幼苗在响应低温胁迫生理生化指标上的差异。结果表明:5份种质在响应胁迫的生理生化指标上存在明显差异,其中骨粒青较耐低温、南农513不耐低温,而且耐低温种质在抗氧化胁迫、光合作用及细胞膜的稳定性上优于不耐低温种质。抗氧化酶活性、光合速率及光响应曲线能够判断耐低温种质;相对电导率、丙二醛含量能够判断不耐低温种质。Cold stress adversely affects seed germination and seedling growth of spring soybean in South China. In this study, the biophysiological and biochemical indexes of seedlings of 5 spring soybean germplasms under cold stress were determined, aiming to establish the method for evaluating and screening cold tolerance germplasms. The results indicated that there were siginificant differences in biophysiological and biochemical responses to cold stress among the 5 germplasms. Guliqing was cold tolerant,while Nannong 513 cold sensitive. The cold tolerance germplasms were better in oxidation resistance, photosynthesis and stability of cell membrance than the cold sensitive germplasms. Antioxidase activity, photosynthesis rate and light response curve could be used as the indexes for screening cold tolerant germplasms, while relative conductivity and MDA content as the indexes for determining cold sensitive germplasms.

关 键 词：大豆 低温胁迫 生理生化指标 评价 

分 类 号：S565.1[农业科学—作物学]