木耳菌糠对元蘑胞外酶活性的影响  

Effect of Waste Material of Auricularia auricular on Hohenbuehelia serotina Extracellular Enzyme Activities

作　　者：邹莉[1] 张国权[1] 孙婷婷[1] 王玥[1] 杨苑艺[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2013年第28期11269-11271,11324,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省博士后启动基金资助(LRB 1111762)

摘　　要：[目的]筛选利用木耳菌糠栽培元蘑的最佳配方。[方法]设计4种配方,选取元蘑菌丝生长期、原基形成期和子实体发育期等阶段,测定其羧甲基纤维素酶(CMC)、半纤维素酶(HC)、淀粉酶(AMY)、漆酶(LAC)4种胞外酶的活性。[结果]在不同配方中,元蘑菌丝体所分泌的CMC、HC、AMY和LAC酶活具有明显的差异,但同种酶的活性在不同配方中变化趋势基本一致;经过显著性分析,配方1、配方2和配方3胞外酶活性无显著差异,但配方4的酶活显著低于其他3种配方。[结论]在胞外酶水平上,配方1、配方2和配方3用来栽培元蘑都是可行的。从经济成本考虑,配方3(菌糠含量为36%)为最佳配方。[ Objective ] The research aimed to select the optimal formulation to cultivate Hohenbuehelia serotina with waste material of Auricu- laria auricular. [ Method ] 4 formulations were designed and mycetium growth phase, primordium formation phase and fruiting body growth phase were chosen, the activities of CMC, HC, AMY and LAC were determined. [ Result] In different formulations, CMC, HC, AMY and LAC that H. serotina mycelium secreted had significant differences, but the same enzyme activity had the consistent tendency in different for- mulations. After significance analysis, extracellular enzyme activities in formulation 1,2,3 had no significant differences, but they were signifi- cantly lower in formulation 4. [ Conclusion ] On the extracellular enzyme level, cultivating H. serotina with formulation 1,2,3 was feasible. Considering economic costs, formulation 3 （waste material of 36% ） was optimal.

关 键 词：元蘑 木耳菌糠 胞外酶活性 

分 类 号：S646[农业科学—蔬菜学]