野生黄蝉兰无菌快繁技术的研究  

In vitro Mass Scale Propagation of Wild Cymbidium iridioides D. Don

作　　者：王玉英[1] 李光宏 李志敏 李枝林[1,3] 

机构地区：[1]云南农业大学园林园艺学院花卉研究所,云南昆明650201 [2]大理兰国花业发展有限公司,云南大理671003 [3]生物多样性与云南特色农业协同创新中心,云南昆明650201

出　　处：《安徽农业科学》2013年第28期11275-11277,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30160074);云南省自然科学基金重点项目(2002C0003P);云南省重点新产品开发项目(2012BB008)

摘　　要：[目的]建立野生黄蝉兰的无菌快繁技术体系。[方法]以云南野生黄蝉兰为试材，以自交果实为外植体，通过在1／2MS基本培养基中，添加不同浓度的BA和NAA以及附加物质香蕉泥和活性碳进行培养，构建野生黄蝉兰的非共生萌发和快繁技术体系。[结果]野生黄蝉兰在2．0～2．5mg／LBA与0．5～1．0mg／LNAA的激素组合处理下，40d后种子萌发率可达93％；1／2MS＋2．5mg／LBA＋0．1mg／LNAA＋浓度8％香蕉泥培养的野生黄蝉兰丛芽增殖率为320％；1／2MS＋0．3mg／LNAA＋0．3％活性炭诱导生根率达100％，且植株长势正常。[结论]通过植物组织培养技术，成功构建了野生黄蝉兰的非共生萌发和快繁技术体系。[ Objective ] To set up the technical system of tissue culture and rapid propagation of wild Cymbidium iridioides D. Don. [ Method] With Yunnan wild Cymbidium iridioides D. Don as test material, self - pollination were used as explants in tissue culture to study the effects of BA and NAA in different concentrations, mashed bananas and activated carbon on their regeneration under 1/2MS basic medium. The system of asymbiotic seed germination and rapid propagation of wild Cymbidium iridioides D. Don was ~onstructed. [ Result ] Combination of BA 20. - 2.5 mg/L and NAA 0.5 - 1.0 mg/L can make seed germination rate to 93%, 1/2MS ＋ BA 2.5 mg/L ＋ NAA 0.1 mg/L ＋ 8% mashed ba- nanas can make multiplication rate to 320%, 1/2MS ＋ NAA 0.3 mg/L ＋0.3% active carbon can make rooting rate to 100% and plant grow healthily. [ Conclusion] The system of asymbi0tic seed germination and rapid propagation of wild Cymbidium iridioides D. Don was constructed successfully by the technology of plant tissue culture.

关 键 词：野生黄蝉兰(Cymbidium iridioides D Don) 种子 快繁技术 

分 类 号：S682.1[农业科学—观赏园艺]