丛柳珊瑚Euplexaura rhipidalis中细胞毒活性二萜成分分析  

Analysis of Cytotoxic Diterpenoid Composition from the Gorgonian Coral Euplexaura rhipidalis

作　　者：孙雪萍[1,2] 任义[3] 徐艳[1] 童万平[1] 王长云[2] 

机构地区：[1]广西海洋研究所海洋生物技术重点实验室,广西北海536000 [2]中国海洋大学医药学院海洋药物教育部重点实验室,山东青岛266003 [3]北海艺术设计职业学院环境艺术设计系,广西北海536000

出　　处：《广西科学》2013年第4期269-271,275,共4页Guangxi Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻11107011-8);广西科学研究与技术开发计划(桂科攻11107011-6)项目资助;广西科学院基本科研业务费项目(13YJ22HYS15)

摘　　要：为了寻找结构新颖的海洋活性成分,对一种采自中国西沙海域丛柳珊瑚Euplexaura rhipidalis进行分离和纯化,并对获得的西松烷二萜化合物的抗肿瘤活性进行筛选,以探明该种丛柳珊瑚的活性组成成分。先利用正反相硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法进行分离和纯化,再利用NMR、MS等波谱方法,并与文献对照确定了3个高氧化度的西松烷二萜类化合物,分别为corulloidolide A(1),(1 R,3 S,4 S,7 E,11 E)-3,4-epoxycembra-7,11,15-triene(2),(-)-corulloidolide E(3);细胞毒活性模型评价结果发现,化合物3表现了较强的细胞毒活性,对5种癌细胞肝癌HepG2,Hep3B,乳腺癌阿霉素耐药株MCF-7/ADR,前列腺癌PC-3he,结肠癌HT-116的IC50值都低于10μg/mL。表明西松烷二萜类化合物是该丛柳珊瑚E.rhipidalis的主要活性化合物类型之一。The chemical constituents and their antitumor activities of Chinese gorgoman coral Euplexaura rhipidalis were preliminarily investigated in order to find more novel marine active ingredients.The ethanolic extract of E.rhipidalis was separated by repeated silica gel column chromatography,Sephadex LH-20 column chromatography and HPLC,by which three compounds were afforded.They were elucidated as three highly oxidized cembranoid diterpenoids：corulloidolide A （1）,（1R,3S,4S,7E,11E）-3,4-epoxycembra-7,11,15-triene （2） and （-）-corulloidolide E （3） by spectroscopic methods （NMR,MV） and by comparing with literatures.Compound 3 showed strong cytotoxicity to five strains of cancer cells including HepG2,Hep3B,MCF-7/ADR,PC-3he and HT-116 with the IC50 of less than 10 μg/mL.It was proposed that cembranoid diterpenoids should be one kind of the bioactive substances of gorgonian coral Euplexaura rhipidalis.

关 键 词：珊瑚 西松烷二萜 细胞毒活性 

分 类 号：Q657.63[生物学—生物物理学] R284[医药卫生—中药学]