方格星虫多糖水法提取的条件及优化  

Optimization of Water Extraction for Polysaccharide fromSipunculus nudus Linnaeus

作　　者：许明珠 张琴 童潼 董兰芳 于海瑞[2] 

机构地区：[1]广西壮族自治区海洋研究所广西海洋生物技术重点实验室,广西北海536000 [2]潍坊学院生物与农业工程学院,山东省高校生物化学与分子生物学重点实验室(潍坊学院),潍坊高新区天润生物工程研发中心,山东潍坊261061

出　　处：《广西科学》2013年第4期285-288,共4页Guangxi Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻11107011-6);广西科学院基本科研业务费项目(12YJ25HYS20)资助

摘　　要：分别从料液比、浸提时间、浸提次数、浸提温度等4个方面初步研究水法提取方格星虫体壁中的水溶性多糖的条件及优化。在单因素试验结束后,通过正交试验取四因素三水平得到方格星虫水溶性多糖水提法的最佳组合。结果:影响方格星虫多糖提取率的主次顺序为浸提温度>料液比>浸提时间>浸提次数,最佳的浸提条件为:温度100℃,料液比1∶12g/mL,浸提时间3h,浸提次数4次。在最优浸提条件下,方格星虫水溶性多糖水提法所得的最佳提取率为1.22%。该工艺稳定可行,能有效提高方格星虫多糖的提取率。The aim of this study is to choose the optimum water extraction process of water soluble polysaccharide from Sipunculus nudus Linnaeus.The orthogonal test design with four factors was used to study the effect of the ratio of material to fluid,extraction time,extraction temperature and extraction times on the extracting rate of water soluble polysaccharides from S.nudus.The factors that affected polysaccharide yield from S.nudus ranked as extraction temperature 〉 ratio of material to fluid 〉 extraction time 〉 extraction times.The optimum extraction conditions were temperature of 100℃,ratio of material to fluid of 1 ∶ 12 g/mL,time for 3h and extraction for 4 times.Under the above conditions,the optimum extraction rate reached 1.22%.This extraction technology is stable and practical with high extraction rate.

关 键 词：方格星虫 多糖 水提 正交试验 

分 类 号：R284.2[医药卫生—中药学]