2种方法制备硫酸化小刺猴头菌多糖  

Two Methods to Prepare Sulfated Polysaccharides from Hericium caputmedusae

作　　者：王新宇[1,2] 迟祥[3] 石亮[3] 沈思捷[2] 王雪 宋慧[2] 

机构地区：[1]白求恩医科大学制药厂,长春130012 [2]吉林农业大学生命科学学院,长春130118 [3]长春生物制品研究所,长春130062 [4]大连珍奥药业有限公司,大连116620

出　　处：《吉林农业大学学报》2013年第6期704-707,共4页Journal of Jilin Agricultural University

基　　金：国家科技支撑计划项目(2007BAI38B06-03)

摘　　要：采用氯磺酸—吡啶法和浓硫酸法制备硫酸化小刺猴头菌多糖,傅立叶变换红外光谱鉴定硫酸化多糖。结果表明:修饰后的多糖在1 259.17 cm-1和807.73 cm-1或1 226.44 cm-1处出现硫酸酯键吸收峰,表明硫酸基团已经与多糖结合成酯。采用氯化钡—明胶法测定硫酸根含量,制备取代度为0.94或0.82的硫酸化多糖。Sulfated Hericium caput-medusae polysaccharides were prepared through wolfrom and sulfuric acid methods and the obtained polysaccharides were characterized by Fourier-transform infrared spectra. The results showed the polysaceharide emerged absorption peak corresponded to sulfate ester at 1 259.17 cm^-1 and 807.73cm^-1 or 1 226.44 cm^-1 after modification, indicating the group of sulphuric acid had conjugated ester with polysaccharide. The content of sulphate was determined through barium- chloride gelatin procedures. Sulfated polysaecharides with substitution degrees of 0.94 and 0.82 were prepared.

关 键 词：小刺猴头菌 多糖 氯磺酸-吡啶法 浓硫酸法 氯化钡-明胶法 

分 类 号：R282.71[医药卫生—中药学]