小刺猴头菌丝体多糖的纯化及性质  

Purification and Characteristics of Polysaccharides for the Mycelium of Hericium caput-medusae

作　　者：杨扬[1] 綦天华 胡静[1] 杜金[1] 吴宗翰[1] 宋慧[1,3] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]长春市水产研究院,长春130033 [3]吉林农业大学食药用菌教育部工程研究中心,长春130118

出　　处：《吉林农业大学学报》2013年第6期708-715,共8页Journal of Jilin Agricultural University

基　　金：吉林省世行贷款农产品质量安全项目(2011-Y18)

摘　　要：试验研究了小刺猴头菌丝体多糖的结构性质、抗氧化活性和抗肿瘤活性。对小刺猴头菌丝体采用水提和碱提2种方法获得粗多糖,进行DEAE-cellulose,SephadexG-75和Sepharose CL-6B柱层析进一步纯化,获得的多糖组分经纯度检验之后进行多糖性质的研究。结果表明:小刺猴头菌丝体水提多糖主要含有葡萄糖和甘露糖;碱提多糖主要含有葡萄糖和半乳糖;小刺猴头菌丝体多糖具有一定的抗氧化活性和抗肿瘤活性。Polysaccharides for the mycelium of Hericium caput-medusae were studied on the structural properties, antioxidant activity and anti-tumor activity. Polysaccharides for the mycelium of Hericium ca- put-medusae were extracted by water extraction and alkali extraction. The coarse polysaccharides were pu- rified by DEAE-cellulose, Sephadex G75 and Sepharose CL-6B gel column chromatography methods. The pure polysaccharide was studied on properties after the purity test. The results showed that water extraction polysaccharides for the mycelium of Hericium caput-medusae mainly contained glucose and mannose; alka- li extraction polysaccharides mainly contained glucose and galactose. Polysaccharides for the mycelium of Hericium caput-medusae have certain antioxidant activity and antitumor activity.

关 键 词：小刺猴头菌丝体多糖 分离纯化 抗氧化 抗肿瘤 

分 类 号：R282.71[医药卫生—中药学]