IGFBP-3基因-202A/C单核苷酸多态性与膀胱癌发生的相关性研究  

ASSOCIATION BETWEEN IGFBP-3-202A/C SINGLE NUCLEOTIDE POLYMORPHISM AND BLADDER CANCER

作　　者：王魏龙 唐良友[1] 王定勇[1] 

机构地区：[1]四川省绵阳市人民医院泌尿外科,四川绵阳621000

出　　处：《河北医科大学学报》2013年第12期1521-1524,共4页Journal of Hebei Medical University

摘　　要：目的探讨胰岛素样生长因子结合蛋白3（insulin—like growth factor binding protein-3，IGFBP-3）基因-202A／C多态性与膀胱移行细胞癌危险性的关系。方法本研究为以中国四川人群为研究对象的病例对照研究，选取98例膀胱癌患者为病例组，150例性别、年龄相匹配的健康者为对照组。应用聚合酶链反应-限制性片段长度多态性分析方法对单核苷酸多态性（rs2854744）进行基因分型。结果病例组和对照组IGFBP-3-202A／C位点基因型及等位基因差异有统计学意义（X2=12．965，P=0．002；X2=11．545，P=0．001）。携带AA基因型发生膀胱癌的风险是cc基因型的0．224倍（OR=0．244，95％CI=0．109—0．549，P：0．001），携带等位基因A是等位基因C的0．531倍（0R=0．531，95％CI=0．368—0．766，P=0．001）。基因型AA和AC较基因型CC发生浸润性膀胱癌风险显著降低（D尺=0．211，95％CI=0．081～0．548，P=0．001；OR=0．150，95％CI=0．036～0．631，P=0．010），携带等位基因A发生浸润性膀胱癌风险是携带等位基因C的0．363倍（OR=0．363，95％CI=0．191～0．691，P=0．002）。结论IGFBP-3-202A／C多态性可能是膀胱癌的危险因素。Objective To investigate the relationship between polymorphism （rs2854744） at - 202A/C in the insulin-like growth factor （IGF）-binding protein-3 （IGFBP-3） gene and the risk of bladder cancer. Methods In a hospital based case-control study for the Chinese population in Sichuan, 98 patients with bladder cancer and 150 age- and sex- matched healthy subjects were enrolled. One single nucleotide polymorphism （rs2854744） was genotyped by polymerase chain reaction-restriction fragment length polymorphism （PCR-RLFP） technique. Results We found statistically significant differences in the genotypic distribution and allele frequency of IGFBP-3-202A/C between the cases and the control subjects （ X2 = 12. 965, P = 0. 002 ; X2 = I 1. 545, P = 0.001 ）. We detected significantly decreased risk of bladder cancer associated with AA genotype （ OR = 0. 244,95% CI = 0. 109 - 0. 549 ; P = 0. 001 ） compared with the CC genotype. Compared to the individual carrying allele C, the risk of bladder cancer with allele A decreased 0. 531 times （ OR = 0.531,95% CI = 0.368 - 0. 766 ,P = 0.001 ）. We detected significantly decreased risk of invasive bladder cancer associated with AA and AC genotype （OR = 0.211,95% CI=0.081 -0.548,P =0.001;OR =0. 150,95% CI=0.036-0.631,P=0.010） compared with CC genotype. Compared to the individual carrying allele C, the risk of invasive bladder cancer with allele A decreased 0. 363 times （ OR = 0. 363,95% CI = 0. 191 - 0. 691, P = 0. 002）.Conclusion IGFBP-3-202A/C gene polymorphism may be a risk factor for bladder cancer.

关 键 词：膀胱肿瘤 胰岛素样生长因子结合蛋白3 多态性 单核苷酸 

分 类 号：R694[医药卫生—泌尿科学]