何首乌饮对衰老大鼠卵巢组织凋亡相关蛋白FOX、SIRT1和c-Myc的调节作用  

Effects of Heshouwuyin on the expressions of apoptosis- associated genes FOX, SIRT1 and c- Myc protein in the ovary of the aging rats

作　　者：张明明[1] 贾晓梅[1] 王超[1] 高伟[1] 彭克楠 赵明[1] 张翠改[1] 李亚丽[1] 

机构地区：[1]河北省人民医院,石家庄050051

出　　处：《医学动物防制》2013年第12期1368-1370,共3页Journal of Medical Pest Control

基　　金：河北省青年科技基金(08028)

摘　　要：目的观察分析对衰老大鼠给予何首乌饮后大鼠卵巢组织凋亡相关蛋白FOX、SIRT1、c-Myc表达的影响。方法大鼠给予D-半乳糖连续腹腔注射法制备亚急性衰老大鼠模型,模型制备成功后何首乌饮连续灌胃60d,大鼠断头取卵巢。采用Western Bloting法检测各组大鼠卵巢FOX、SIRT1及c-Myc蛋白表达的变化。结果模型组较正常组卵巢细胞FOX、SIRT1及c-Myc表达量降低;何首乌饮可使卵巢组织FOX、SIRT1及c-Myc表达量增加,并呈现一定的剂量依赖性,以高剂量最佳,差异有统计学意义(P<0.05)。结论何首乌饮方剂能延缓衰老大鼠卵巢细胞凋亡的发生,其机制可能是通过干预凋亡相关基因FOX、SIRT1及c-Myc蛋白表达抑制卵巢细胞的凋亡。Objective To investigate the effects of Heshouwuyin （HSWY） on ovary apoptosis and expression of associated genes FOX, SIRT1 and c - Myc protein in aging rats. Methods The model of sub - acutely aging rats was made by injecting D - gal continually into the SD rats＇abdominal cavity. After the model was comple- ted, HSWY was given to these rats for 60 days. Then Western - bloting were applied to observe FOX, SIRT1 and e - Myc protein of ovary. Results The expression of FOX, SIRT1 and c - Myc protein was lower in ovary cells of model group than in normal group. The expression of FOX, SIRT1 and c - Myc protein in ovary cells of model group increased after treated with Hswy. Hswy could improve the expression of FOX, SIRT1 and c - Mye in ovary. （ P 〈0.05 ） ; The result takes on a dose - effect relationship, and the effect of high dose group is better. Conclusions The HSWY can reduce the incidence of ovary apoptosis , and its anti - aging role may- be regulate the expression of FOX, SIRT1 and e -Mye and blocking apoptosis.

关 键 词：何首乌饮 卵巢 凋亡 凋亡相关基因 

分 类 号：R285[医药卫生—中药学] R339.38[医药卫生—中医学]