阿维A对硬皮病小鼠的治疗作用及机制研究  

The therapeutical effect and mechanism of acitretin in scleroderma mice model

作　　者：沈佚葳[1] 王京[2] 周平[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院皮肤科,北京100038 [2]首都医科大学附属北京世纪坛医院中心实验室,北京100038

出　　处：《中国医药导报》2013年第25期20-22,共3页China Medical Herald

基　　金：首都医科大学附属北京世纪坛医院国自然青年博士基金项目(编号2012-QB12)

摘　　要：目的研究阿维A(acitretin)对系统性硬皮病(SSc)小鼠皮肤及肺部纤维化的治疗作用,并通过检测小鼠外周血Th17/Treg相关因子白介素(IL)-17、IL-10、IL-6、转化生长因子(TGF)-β1水平,探讨阿维A治疗硬皮病可能的机制。方法 24只雌性BALB/c小鼠随机分为对照组、硬皮病组和阿维A组,硬皮病组和阿维A组给予博莱霉素皮下注射4周,阿维A组同时每天给予6 mg/kg的阿维A灌胃4周。4周后取注射部位皮肤及肺部制作组织病理切片,观察小鼠皮肤厚度及皮肤/肺部炎症。酶联免疫吸附法(ELISA)检测小鼠血清IL-17、IL-10、IL-6、TGF-β1水平。结果阿维A组皮肤厚度、皮肤炎症评分、肺部炎症评分均明显低于硬皮病组[(140.25±20.24)μm比(191.88±40.95)μm,(0.75±0.01)分比(2.00±0.76)分,(1.38±0.52)分比(2.00±0.92)分,P<0.05]。阿维A组血清IL-17、IL-6、IL-10均显著低于硬皮病组[(50.69±11.72)pg/mL比(66.89±16.62)pg/mL,(85.77±12.06)pg/mL比(118.68±22.62)pg/mL,(57.65±21.74)pg/mL比(131.07±22.52)pg/mL,P<0.05]。三组小鼠血清TGF-β1水平差异无统计学意义。结论阿维A可显著改善硬皮病小鼠皮肤及肺部症状,其作用机制可能与影响Th17/Treg的平衡有关。Objective To study the therapeutical effect of acitretin for systemic scleroderma （SSc） in the mouse model.Peripheral blood IL-17,IL-10,IL-6 and TGF-[β1,which were Thl7/Treg related factors were examined to explore the possible mechanism of acitretin.Methods 24 female BALB/c mice were randomly divided into control group,SSc group and acitretin group.SSc group and acitretin group were given subcutaneous injections of bleomycin for 4 weeks,and acitrein group were given acitretin 6 mg/（kg· d） orally for 4 weeks.4 weeks later,pathological changes of the skin and lung of the mice were detected.Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of IL-17,IL-10,IL-6 and TGF-[β1 in the serum.Results Skin thickness,skin inflammation score,lung inflammation score of acitretin group were significantly lower than the SSc group [（140.25±20.24） μm vs （191.88±40.95） pm,（0.75±0.01） scores vs （2.00±0.76） scores,（1.38±0.52） scores vs （2.00±0.92） scores,P ＜ 0.05）.Serum IL-17,IL-6,IL-10 of acitretin group were significantly lower than the SSc group [（50.69±11.72） pg/mL vs （66.89±16.62） pg/mL,（85.77±12.06） pg/mL vs （118.68±22.62） pg/mL,（57.65±21.74） pg/mL vs （131.07±22.52） pg/mL,P ＜ 0.05].Serum TGF-β1levels of three groups showed no significant differences.Conclusion Acitretin significantly improves symptoms of skin and lungs in the scleroderma mice model,and its mechanism may related with Th17/Treg balance.

关 键 词：硬皮病 阿维A IL-17 IL-6 IL-10 

分 类 号：R593.25[医药卫生—内科学]