16S rDNA对四种常见细菌中的鉴定与应用  

Application and Identification in Four Kinds of Common Bacteria by 16S rDNA

作　　者：张伟信[1] 邬晓凤 喻华英[1] 

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔市843300

出　　处：《塔里木大学学报》2013年第4期7-11,共5页Journal of Tarim University

基　　金：国家自然基金(31060344)

摘　　要：本实验利用16S rDNA序列分析技术,对来自犊牛腹泻粪便中的四株菌(A菌,B菌,C菌,D菌)进行菌种鉴定。使用16S rDNA通用引物,通过PCR扩增分别得到1 500 bp,与GenBank中序列进行比对。结果表明:A菌与肠杆菌属、B菌与短小芽孢杆菌、C菌与大肠杆菌、D菌与变形杆菌的同源性最高,相似率均达到了99%,初步确定A菌为肠杆菌属、B菌为短小芽孢杆菌、C菌为大肠杆菌、D菌为变形杆菌。本试验的目的是为16S rDNA在临床细菌的鉴定提供客观理论依据。This experiment used sequence analysis of 16SrDNA for four bacteria （ A, B, C, D） from calf diarrhea feces. The test used PCR, amplified fragment was 1 500 bp by 16S rDNA universal primers. The sequence blasted with Gene Bank. The results showed that： the A bacteria was the Enterobacter sp, the B bacteria was the Bacillus pumilus strain, the C bacteria was the Escheriehia coli and the D bacteria was the Proteus vulgaris, the similarity ratio was reached 99% , initially that the A bacteria identified the Enter- obacter sp, the B bacteria identified the Bacillus pumilus strain, the C bacteria identified the Escherichia coli and the D bacteria iden- tified the Proteus vulgaris. The experiment aimed to provided the theoretical basis of the identification clinical bacteria by 16S rDNA.

关 键 词：16S RDNA 细菌 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]