嗜水气单胞菌和大肠杆菌LPS对草鱼免疫相关基因表达的影响  

Effects of LPS fromAeromonas hydrophila and Escherichia coli on expression of immune related genes in grass carp,Ctenopharyngodon idella

作　　者：彭小云[1] 刘红[1] 陈孝煊[1] 张棋[1] 袁娟[1] 刘佳佳[1] 吴志新[1] 

机构地区：[1]华中农业大学水产学院/农业部淡水生物繁育重点实验室/淡水水产健康养殖湖北省协同创新中心,武汉430070

出　　处：《华中农业大学学报》2014年第1期67-72,共6页Journal of Huazhong Agricultural University

基　　金：国家科技支撑计划项目(2012BAD25B05;2012BAD25B06);公益性行业(农业)科研专项(200803013);中央高校基本科研业务费专项(2013PY070)

摘　　要：将嗜水气单胞菌粗脂多糖(C-LPS)和大肠杆菌脂多糖(E-LPS)分别经腹腔注射草鱼(剂量为4mg/kg),以注射PBS为对照。48h后,分别提取各组草鱼的头肾、脾脏、肾脏、肝脏和肠组织中的总RNA,利用Real-time PCR的方法检测不同组织中TNF-α、IL-1β、IL-10和TLR4基因的表达情况。结果显示,与对照组相比,头肾中,TNF-α和IL-1β的表达量在E-LPS组显著升高,而IL-10的表达显著下降;C-LPS组TNF-α和IL-10的表达量显著性升高;TLR4在两试验组均有显著性降低。脾脏中,TNF-α、IL-1β和IL-10在E-LPS组与对照组无显著差异,而IL-1β和IL-10的表达在C-LPS组有显著性升高;TLR4在两试验组有显著性降低。肾脏中,TNF-α表达在E-LPS组有显著性升高;IL-1β和IL-10在C-LPS组有显著性升高;TLR4在两试验组中与对照组无显著性差异。肝脏中,TNF-α、IL-10和TLR4在两试验组均有显著性升高;IL-1β的表达在E-LPS组显著高于对照组和C-LPS组。肠组织中,TNF-α和IL-10在两试验组有显著性降低和升高;IL-1β和TLR4与对照组间无显著性差异。表明嗜水气单胞菌C-LPS和大肠杆菌LPS均可引起草鱼细胞因子基因表达的变化,对于机体免疫反应起到一定的调节作用,但LPS对TLR4基因并没有引起显著的上调作用,这与哺乳类动物的研究结果不同。In the present study, grass carps were injected intraperitoneally with crude Aeromonas hydrophila lipopolysaccharide （C-LPS）,pure Escherichia coli LPS （E LPS） and phosphate-buffered sa- line （PBS, as control） at 4 mg/kg, respectively. After 48 h, fish tissues including head kidney, spleen, liv- er,kidney and intestine in the treated and control group were taken to detect the expression of TNF-α, IL-1β, IL-10 and TLR4 genes by quantitative real time PCR. The results showed that,in head kidney, the expression of TNF-α and IL-1β genes was significantly up-regulated, but the expression of IL-10 was significantly decreased in E-LPS group; the expression of TNF-α and IL-10 genes were significantly up- regulated in C-LPS group, and the TLR4 was significantly decreased in both treated groups. In spleen, the expression of TNF-α, IL-1β and IL-IO genes was not significantly different in E-LPS group from the control, the IL-1β and IL-10 levels were significantly up-regulated in C-LPS group, and the expression of TLR4 was significantly decreased in both treated groups. In kidney, the TNF-α and IL-1β, ILl0 levels were significantly increased in the two treated groups, but the expression of TLR4 gene was not different from the control. In liver, the TNF-a, IL-10 and TLR4 levels were significantly higher in the two treated groups, while the expression of IL-1β was significantly up-regulated in E-LPS group compared to both the control and C-LPS groups. In intestine, the TNF-a and IL-10 genes were significantly induced in the two treated groups and the IL- 1β and TLR4 genes were not significantly induced. These results indicated that LPS can induce the expression of the immune related genes of the host, but LPS could not make the up-regulation of the TLR4 gene,which is different from the mammal.

关 键 词：嗜水气单胞菌 大肠杆菌 脂多糖 草鱼 免疫相关基因 

分 类 号：S942.1[农业科学—水产养殖]