理化因素刺激对牙髓及其胶原影响的实验研究  

Effect of physical and chemical factors on Macaca Mulatta′s pulp and change of the collagen in pulp

作　　者：袁理[1] 岳林[1] 毛秀萍[1] 高岩[2] 

机构地区：[1]北京大学口腔医学院牙体牙髓科,100081 [2]北京大学口腔医学院病理科,100081

出　　处：《中华口腔医学杂志》2001年第1期52-54,T002,共4页Chinese Journal of Stomatology

摘　　要：目的　了解猴牙髓及其胶原对物理、化学刺激的反应。方法　观察磷酸锌粘固剂及氧化锌丁香油酚粘固剂 (zincoxideeugenol,ZOE)间接盖髓后 ,恒河猴牙髓即刻、3d、7d、1个月、3个月HE和胶原特殊染色后的组织学变化。结果　①磷酸锌粘固剂组术后 7d～ 3个月 ,牙髓以局限性炎症损伤为主 ;②ZOE组术后 1～ 3个月胶原减少明显下降 ,已无炎症细胞浸润 ;③胶原特殊染色法可清楚地反映牙髓胶原的形态特点、分布及变化 ,术后 3d胶原明显减少早于炎症细胞的出现。结论　磷酸锌粘固剂对牙髓产生化学刺激作用 ;ZOE可促进损伤牙髓的修复 ;胶原可作为更敏感的指标 ,指示牙髓的损伤和修复。Objective To investigate the change of pulp and its collagen to outside stimuli. Methods 51 permanent teeth from 10 adult healthy Macaca Mulatta′s monkeys were chosen as experimental teeth. 39 of them were prepared Class V cavities. The teeth were divided into two groups, one was filled with zinc phosphate cement and another group with zinc oxide eugenol. A blank control group of 12 teeth was set at the same time. Just after the operation, 3 d, 7 d, 1 month and 3 months later, the animals were executed. Histologic changes were evaluated by means of HE and collagen staining method. Results Local inflammation was the major representation in the pulps of the zinc phosphate cement group from 7d to 3 months after operation. The percentage of collagen decrease dropped sharply,and there was no inflammation cells infiltration in the pulps of zinc oxide eugenol group from 1 to 3 months after operation. By means of collagen staining method, the morphological characters ,distribution and the change of pulpal collagen during a certain pathologic process were clearly represented in histologic slices. The change of collagen appeared earlier than the inflammation cells infiltration. Conclusions Zinc phosphate cement can lead to medium inflammation of the pulp. The change of collagen appears earlier than that of WBC, which can be used as a sensitive indicator.

关 键 词：牙髓 胶原 磷酸锌粘固剂 氧化锌丁香油酚粘固剂 理化因素刺激 

分 类 号：R312[医药卫生—基础医学]