MHCI类抗原多肽致敏IL-2基因修饰的树突状细胞对小鼠引流淋巴结T细胞亚群的影响及其免疫保护作用  

The affection to lymphocyte subsets in the draining lymphoid node and immune protected effect from the MHC class I antigen peptides pulsed DCs with IL-2 gene modified immunized mice

作　　者：孙黎飞[1] 曹雪涛[1] 刘海军[1] 

机构地区：[1]第二军医大学免疫学教研室,上海200433

出　　处：《中国免疫学杂志》2001年第1期13-15,18,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金 !(39730 42 0 )资助

摘　　要：目的 :探讨MHCI类分子限制性肿瘤抗原多肽Mutl体外冲击白细胞介素 2 (Interleukin 2 ,IL 2 )基因修饰的树突状细胞 (Dendrticcells,DC)对小鼠体内特异性免疫的活化机制。方法 :用腺病毒作为载体介导小鼠IL 2基因修饰DC ,用小鼠Lewis肺癌 3LL细胞株MHCI类分子限制性八肽Mutl冲击IL 2基因修饰的DC(DC IL 2 Mutl)免疫小鼠 ,用流式细胞术 (FACS)分析免疫保护小鼠接受 3LL细胞再攻击后引流淋巴结内T细胞亚群的比例变化。结果 :用Mutl冲击的DC免疫的小鼠抵抗3LL细胞再攻击时 ,引流淋巴结内CD8+ T淋巴细胞比例明显升高 ,DC IL 2 Mutl免疫保护的小鼠接受 3LL细胞再攻击后引流淋巴结内CD8+ 和NK细胞的比例都明显升高。结论 :研究表明MHCI类分子限制性肿瘤抗原多肽体外冲击IL 2基因修饰的DC免疫小鼠 ,能诱导小鼠产生特异性抗肿瘤免疫应答 。Objective:To investigate the specific immune activated effects in the mouse immunized by MHC I restriced tumor antigen peptide pulsed dendritic cells(DC) with IL 2 gene modified.Methods:DC were transfected with IL 2 gene via adenovirus vector(DC IL 2) the mRNA of mIL 2 was examined by RT PCR.Syngeneic mice were immunized by DC IL 2 pulsed with MHC I restricted Mutl tumor antigen peptiede of 3LL Lewis lung carcinoma (DC IL 2 Mutl),the effects of lymphocyte subsets in the draining lymphoid node from the tumor antigen pulsed DCs with gene modified immunized mice by flow cytometric analysis(FACS) analysis.Results:The mRNA of mIL 2 was expressed in the DCs.The ratio of CD8 +T cells was hosted in the draining lymphoid node from Mutl pulsed DCs(DC Mutl) immunized mice.The ratio of CD8 +T cells and NK cells were all hosted obviously in the draining lymphoid node from DC IL 2 Mutl immunized mice .Conclusion:Immunized mice with DC IL 2 Mutl can induce the mouse specific antitumor immunized affect in vivo and can activate a variety of immunized effect in the mouse.

关 键 词：树突状细胞 白细胞介素2 基因修饰 MHCI类多肽 T细胞亚群 小鼠 

分 类 号：R392.1[医药卫生—免疫学]