云南西部彝族HLA-DQA1基因构成及中华民族19个华人群体DQA1基因频率的比较分析  

PCR-RFLP polymorphism of HLA-DQA1 in Yichu of north Yunnan province and compared with other Chinese groups

作　　者：潘德京[1] 黄洪莲[1] 刘泽寰[1] 陈为民[1] 林蒋海[1] 陶洪[2] 陈荣祥 吴文言[1] 徐安龙[4] 

机构地区：[1]中山大学生命科学学院,广州510275 [2]云南省卫生防疫站寄防科,昆明650032 [3]云南澜沧县第一人民医院检验科,澜沧665600 [4]广州中山大学生命科学学院生化系,广州510275

出　　处：《中国免疫学杂志》2001年第1期20-23,共4页Chinese Journal of Immunology

基　　金：国家杰出青年基金资助项目!(批准号 :3972 5 0 0 7)

摘　　要：目的 :探讨云南漾濞彝族族群HLA DQA1频率分布特征 ,并与华人 19个群体比较。方法 :采用限制性片段长度多态性分析 (PCR RFLP)方法。结果 :DQA1 0 30 1(31 6 3 % )最高 , 0 40 1(1 0 2 % )最低。卡方总体检验表明 ,该族群与北方六个汉人群体、满族、新加坡群体及新疆维吾尔族无显著差异 (P >0 0 5 ) ,但有个别基因频率的显著差异 ;布依族、傣族、台湾群体组成复杂 ,与其他群体有很大显著差异 (P >0 0 0 1) ;广东汉人与广西壮族与其他群体也有极其显著的差异 ;哈萨克族与维吾尔族相似 ;维吾尔族与部分北方汉族并无显著差异 ;藏族与哈萨克族、新加坡群体无显著差异。结论 :南方各族群DQA1的复杂构成 ,可能与起源的多样性或地域环境有关。漾濞彝族族群HLAObjective:To investigate HLA DQA1 alleles distribution in the Yichu of Yangbi county, Yunnan province, compared with other Chinese group Methods:PCR RFLP.Results: Of 8 DQA1 alleles studied, DQA1*0301 (allele frequency 31 63%) is the most common allele in this Yichu, and DQA1*0401 (1 02%) is the rarest Chi square test shows that Yangbi Yichu has no significant difference with six northern Han groups, Man, Singaporean and Uygurs of Xinjiang In contrast, Buyi, Dai and Taiwan Residents is much more complicated with a significant difference from other groups A similar observation was found in Guangdong Han and Guangxi Zhuang Similar to Kazak, Uygurs showed no significant difference with some of northern Han groups Tibetans have no significant difference with Kazaks and Singaporean Conclusion: DQA1 complexity of southern ethnic group is probably result of mutilple origin or stress of different environment Yangbi Yi ethnic group has characteristic of Northern group

关 键 词：PCR-RFLP HLA-DQA1 彝族 中华民族 基因多态性 D 

分 类 号：R392.2[医药卫生—免疫学] Q346.5[医药卫生—基础医学]