胸腺素α1抗体的制备和鉴定  

Preparation and determination of polyclonal antibody to thymosin α1

作　　者：周克夫[1] 章军[1] 陈天圣[1] 徐虹[1] 秦燕[1] 殴阳青[1] 楼士林[1] 刘仁海[1] 柯珍恋[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005

出　　处：《中国免疫学杂志》2001年第1期43-44,47,共3页Chinese Journal of Immunology

基　　金：国家"863"课题资助!(No 819 0 4 0 3)

摘　　要：目的 :制备用于检测转胸腺素基因蓝藻表达产物的特异性抗体。方法 :用提纯的小肽 (2 8个氨基酸 )胸腺素α1与牛血清白蛋白偶联后作为抗原 ,采用皮下多点注射免疫的方法免疫大鼠。经过 3个月的免疫 ,获得抗Tα的多克隆抗体。结果 :用间接酶联免疫吸附 (ELISA)方法 ,测得抗体效价超过 40 96。蛋白印迹 (Westernblot)检测结果显示 ,该抗体能特异性地与胸腺素α1抗原产生明显免疫亲和反应。结论Objective:To prepare specific antibody against Thymosin α1 Methods:Thymosin α1(Tα1,28 peptide) was conjuncted with BSA as immune antigen,rats were immunized and the polycolonal antibody to Tα1 was obtained.Results:With ELISA detection,the titer of antibody was more than 1:4 096;Western blot analysis showed that the antibody can bind with Tα1 specifically.Conclusion:The prepared antibody against Tα1 has good specificity and reactivity and can be used to detect the expression products of transgenic cyanobacteria which expressed Tα1 gene.

关 键 词：胸腺素α1抗体 酶联免疫吸附反应 蛋白印迹 制备 鉴定 

分 类 号：R392－33[医药卫生—免疫学]