番茄转录因子SlWRKY53的分离及生物学功能鉴定  

Isolation and functional characterization of tomato transcription factor SlWRKY53

作　　者：刘畅[1] 牛向丽[2] 刘继恺[1] 唐维[1] 张霞[1] 张凝[1] 李頔[1] 刘永胜[1,2] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室/水力学与山区河流开发保护国家重点实验室,成都610064 [2]合肥工业大学生物与食品工程学院,合肥230000

出　　处：《四川大学学报（自然科学版）》2013年第6期1347-1354,共8页Journal of Sichuan University(Natural Science Edition)

基　　金：教育部博士点优先资助基金项目(20110181130009);国家自然基金面上项目(31171179);国家高技术研究发展计划(863)课题(2007AA10Z100)

摘　　要：构建番茄SlWRKY53的过量表达载体,通过农杆菌介导转入番茄(Solanum lycopersicum)品种Ailsa Craig(AC+),获得转基因阳性植株.定量分析显示,这些转基因株系中SlWRKY53基因表达量显著高于野生型.表型分析显示,转基因植株对盐胁迫抗性强于野生型.对抗病性进行检测,转基因植株抗性稍强,但与野生型相比无明显差异.由此推测番茄SlWRKY53基因的过量表达能够一定程度增强植株抵抗盐胁迫的能力.The overexpression vector containing the full-length coding region of SlWRKY53 gene was constructed and introduced into tomato cv. Ailsa Craig by Agrobacterium-mediated genetic transforma- tion. Real-time PCR revealed a significant increase of SlWRKY53 expression levels in the transgenic lines compared with that of wild-type plants. Further study indicated that these transgenic lines dis- played enhanced tolerance to NaCI treatment. In addition, the transgenic lines were shown slightly en- hanced resistance to Pst DC3000 pathogen inoculation than that of wild-type plants but without signifi- cant difference. Thus, up-regulation of SlWRKY53 in tomato plants might result in enhanced tolerance to salt stress.

关 键 词：番茄 SlWRKY53 过量表达 农杆菌介导转化 抗盐 

分 类 号：Q812[生物学—生物工程]