载脂蛋白E基因多态性与高脂血症的关系  

Relationship between apolipoprotein E gene polymorphism and hyperlipidemia

作　　者：曾志为[1] 彭澍[1] 彭健[1] 龚五星[1] 

机构地区：[1]广东省珠海市人民医院心内科,519000

出　　处：《广东医学》2001年第2期120-121,共2页Guangdong Medical Journal

基　　金：国务院侨务办公室 (部级 )重点学科科研基金立项课题 !(项目编号1 9981 75);广东省医药科研立项资助课题 !(项目编号 1 998567)

摘　　要：目的　研究载脂蛋白E(apoE)基因多态性对血脂水平的影响及其与高脂血症的关系。方法　应用聚合酶链反应结合限制性片段长度多态性 (PCR -RFLP)检测法 ,测定 87例正常对照者和 163例高脂血症患者的apoE基因型 ;血脂水平按常规方法测定。结果　共检测出 4种apoE基因型 ,分别为E3 / 3、E3 / 2、E4/ 3及E4/ 2。高脂血症组apoE4/ 3基因型和ε4等位基因频率均高于对照组 (P <0 0 5 ) ;不同的apoE等位基因间TC和LDL -C水平存在差别 ,依次是ε4>ε3 >ε2。结论　apoE基因多态性影响血脂的代谢水平 ;Objective To study the influence apolipoprotein E(apoE) gene polymorphism on plasma lipid levels and hyperlipidemia. Methods By using PCR-RFLP, we determined apoE genotype of 87 hyperlipidemia patients and 163 healthy controls. Other plasma lipid para-meters were measured by routine method. Results Four genotypes of apoE, E3/3, E3/2, E4/3 and E4/2 were detected in this study. There were statistically significant differences in apoE4/3 and ε4 frequencies between CHD patients and controls ( P <0 05). Hyperlipidemia patients had higher plasma total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) than those of controls ( P <0 05). Among different apoE genotypes, there were statistically significant difference in TC and LDL-C level (ε4>ε3>ε2). Conclusion that apoE gene polymorphism may affect plasma lipid levels. apoE ε4 may be an important genetic factor of hyperlipidemia.

关 键 词：载脂蛋白E 基因频率 高脂血症 基因多态性 

分 类 号：R589.202[医药卫生—内分泌]