不同剂量白细胞介素2作为乙型肝炎病毒核酸疫苗佐剂的效应  

Effects of different doses of interleukin 2 as adjuvants on immune responses induced by nucleic acid vaccines of hepatitis B virus

作　　者：李文波[1] 姚志强[2] 周永兴[2] 权启镇[1] 冯志华 

机构地区：[1]济南军区总医院消化科,山东济南250031 [2]第四军医大学唐都医院感染病科,陕西西安710038

出　　处：《免疫学杂志》2001年第1期27-29,共3页Immunological Journal

基　　金：国家自然科学基金资助项目!(39770 6 6 5 )

摘　　要：目的构建表达乙型肝炎病毒 (HBV) S蛋白的重组质粒 p CR3.1- S作为核酸疫苗 ,观察重组人白细胞介素 2(rh IL - 2 )作为佐剂对其诱导 BAL B/ c小鼠产生免疫应答的影响。方法分别以 EL ISA法、3H- Td R掺入法、Cr5 1 4h释放法等测定免疫小鼠的血清抗 HBs、淋巴细胞增殖活性、淋巴细胞杀伤效应 ,初步研究不同免疫组体液和细胞免疫应答的差异。结果血清抗 HBs EL ISA效价 ,rh IL - 2组与对照组相比较差异不显著 (P>0 .0 5 ) ;淋巴细胞增殖指数、淋巴细胞杀伤率 ,10 0 0 U (只·次 ) rh IL - 2 /组和 2 0 0 0 U (只·次 ) rh IL - 2 /组与对照组相比较差异显著 (P<0 .0 5 ) ,而 5 0 0 U (只·次 ) rh IL - 2 /组与对照组相比较无显著差异 (P>0 .0 5 )。结论一定剂量的 rh IL - 2作为佐剂可增强ObjectiveTo construct recombinant plasmids of pCR3 1 S encoding protein S of hepatitis B virus as nucleic acid vaccines and investigate the effects of recombinant human interleukin 2 as adjuvants on immune responses induced by HBV nucleic acid vaccines in BALB/c mice Methods Anti HBs antibodies to HBV major protein was measured by ELISA assay , and the activities of lymphocyte proliferation and cytotoxity were detected by[KG-2/3] 3H TdR incorporation assay and 4 hour 51 [KG2/3]Cr release assay Humoral and cellular immune responses of different groups were then compared Results The titers of anti HBs antibodies in serum of rhIL 2 group mice were not significantly higher than those of control group mice ( P >0 05). The diffe rence of the activities of lymphocyte proliferation and cytotoxity between of control group mice and rhIL 2 group mice (every mouse with 500 U one time) was not significant ( P >0 05). However, stimulation index and specific lysis rate of lymphocyte of rhIL 2 group mice (every mouse with 1 000 U or 2 000 U one time) showed significant difference compared with htose of control group mice ( P <0 05). Conclusion Recombinant plasmids of pCR3 1 S as HBV nucleic acid vaccines could induced strong humoral and cellular immune responses in BALB/c mice rhIL 2 acts as adjuvants to increase the potency of HBV nucleic acid vaccine in some certainty

关 键 词：白细胞介素2 乙型肝炎病毒 核酸疫苗 佐剂 

分 类 号：R392[医药卫生—免疫学]