大鼠脊髓和后根节内5-HT_(1A,2A)受体mRNA阳性神经元的分布  

DISTRIBUTION OF NEURONS EXPRESSING 5-HT_(1A,2A/) RECEPTOR mRNAs IN THE RAT SPINAL CORD AND DORSAL ROOT GANGLION

作　　者：王文[1] 武胜昔[1] 朱敏[1] 李云庆[1] 

机构地区：[1]第四军医大学基础部解剖学教研室,梁銶琚脑研究中心西安710032

出　　处：《解剖学报》2001年第1期1-4,T001,共5页Acta Anatomica Sinica

基　　金：国家杰出青年科学基金资助项目!( 3 962 5 0 11)

摘　　要：目的　观察大鼠脊髓及后根节内 5 - HT1 A,2 A受体 m RNA阳性神经元的分布。　方法　原位杂交组织化学技术。　结果　 (1) 5 - HT1 A受体 m RNA阳性神经元分布于脊髓灰质各层 ,主要见于后角浅层 ( 、 层 )及 、 层 , ～ 层和 层也有散在分布。前角 ( 层 )内仅有少量阳性神经元 ;(2 ) 5 - HT2 A受体 m RNA阳性神经元的分布较局限 ,主要见于后角浅层及前角 ( 层 )神经元 ,在其他各层仅呈散在分布。在大鼠后根节内观察到 :(1) 10 .4%的后根节细胞呈 5 - HT1 A受体 m RNA阳性 ,阳性细胞以中、小型节细胞为主 ;(2 ) 17.4%的后根节细胞呈 5 - HT2 A受体m RNA阳性 ,阳性细胞也以中、小型节细胞为主。　结论　 5 - HT1 A和 5 - HT2 A受体在脊髓和后根节内具有不同的分布特点 ,它们在介导 5 -羟色胺在脊髓水平的镇痛及在外周的致痛作用中可能扮演着重要的角色。Objective\ To observe the expression of 5\|HT 1A and 5\|HT 2A receptor mRNAs in the rat spinal cord and dorsal root ganglion(DRG). Methods\ In situ hybridization histochemical technique. Results\ (1)5\|HT\-\{1A\} receptor mRNA positive neurons were found in all laminae of the gray matter,mainly in the superficial laminae(laminae Ⅰ and Ⅱ),laminae Ⅲ and Ⅳ of the dorsal horn.Scattered positive neurons were also observed in laminae Ⅴ\|Ⅶ and Ⅹ.Very few positive signals were found in the ventral horn(lamnina Ⅸ);(2)5\|HT\-\{2A\} receptor mRNA positive neurons were mainly found in the superficial laminae and ventral horn,while sparsely distributed positive neurons were also located in other laminae.Within the DRG:(1)About 10\^4% of the total DRG cells were labeled with 5\|HT 1A receptor mRNA.The positive signals were mainly confined to a subpopulation of small\| and medium\|sized cells;(2)About 17\^4% of the total DRG cells were labeled with 5\|HT 2A receptor mRNA,most of them were also small\| and medium\|sized cells. Conclusions\ 5\|HT 1A and 5\|HT 2A receptor mRNAs positive neurons distributed heterogeneously in the rat spinal cord and DRG, they may play important roles for 5\|HT in the analgesic effects at the spinal level and nociception in the periphery.\;

关 键 词：5-HT受全 脊髓 后根节 原位杂交 大鼠 mRNA 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]