大鼠纹状体边缘区内烟碱型乙酰胆碱受体mRNA的表达  

Expression of nicotinic acetylcholine receptor mRNA in the marginal region of rat striatum

作　　者：舒斯云[1] 包新民[1] 张魁华[1] 

机构地区：[1]第一军医大学珠江医院神经科学研究所,广东广州510282

出　　处：《第一军医大学学报》2001年第1期1-4,共4页Journal of First Military Medical University

基　　金：国家自然科学基金!(39770550);广东省科学基金!(950554)

摘　　要：目的研究纹状体边缘区内有无乙酰胆碱受体（ｎＡＣｈＲ）的分布。方法用地高辛标记的 ｎＡＣｈＲ ｃＤＮＡ作为探针， 与大鼠脑切片进行原位杂交。结果大鼠脑纹状体内可见较多的ｎＡＣｈＲ阳性神经元，其中尾壳核中可见较多中等大小 的多极阳性神经元，在尾壳核和苍白球之间的边缘区部位，可见较多阳性梭形细胞，呈密集的带状分布。结论纹状体边 缘区内存在 ｎＡＣｈＲ ｍＲＮＡ的表达，推测边缘区内的 ｎＡＣｈＲ阳性神经元对调节边缘区的学习记忆功能有重要作用。Objective To study the distribution of nicotinic acetylcholine receptor(nAChR) mRNA in the marginal region of rat striatum. Methods In situ hybridization of digoxin-labeled probes on sections of the rat brain was performed. Results Numerous nAChR-Positive neurons were observed in the rat striatum. In the caudoputamen aggregated many multipolar positive neurons of moderate size and a number of fusiform positive neurons were present in the caudoputamen.There were some fusiform positive neurons in the marginal division,which were located in the marginal region between the caudate putamen and globus pallidus. Conclusion The neurons in the marginal region are capable of nACbR mRNA expression, suggesting that nAChR-positive neurons in the marginal region may exercise important functions in the process of learning and memory.

关 键 词：烟碱 纹状体 乙酰胆碱受体 MRNA 大鼠 nRchRcDNA 

分 类 号：R338.26[医药卫生—人体生理学] Q593.2[医药卫生—基础医学]