应用环孢素A治疗异种肝细胞移植的排斥反应  

Therapeutic effects of cyclosporine A on the rejection of xenogenic hepatocyte transplantation

作　　者：赵中辛[1] 刘菲[2] 钟岚[2] 庄志祥[1] 尹华[1] 孙志岭[3] 

机构地区：[1]上海市东方医院普外科,200120 [2]上海市东方医院消化科,200120 [3]南京市第一医院普外科

出　　处：《中华实验外科杂志》2001年第1期50-51,共2页Chinese Journal of Experimental Surgery

基　　金：国家教育部留学回国人员科研基金! (453CC950 1 )

摘　　要：目的　探讨应用环孢素A(CsA)治疗异种肝细胞移植排斥反应的疗效及其排斥机制。方法　将豚鼠肝细胞植入D 氨基半乳糖诱导的肝衰大鼠脾内 ,分CsA治疗组和单纯移植组。观察 2周生存率及脾病理组织学检查 ;酶联免疫双抗体夹心法 (Sandwich ELISA)测定大鼠血清白细胞介素 (IL) 2的浓度。结果　 2周生存率比较 :CsA治疗组 (78.6 % )与单纯移植组 (80 .0 % )相比差异无显著性 (P >0 .0 5 )。应用CsA可减缓炎症细胞浸润。血清IL 2浓度检测 :正常大鼠为(2 8.7± 7.0 )ng/L ,移植后各组血清IL 2浓度变化不大 ,直至移植后第 7天 ,CsA治疗组为 (2 8.5±6 .0 )ng/L、单纯移植组为 (31.5± 8.0 )ng/L。 3组间差异均无显著性 (P >0 .0 5 )。结论　在异种肝细胞脾内移植中 ,细胞免疫发生的较迟和 /或较弱。Objective To investigate the immunosuppressive effect of cyclosporine A (CsA) on xenogeneic hepatocyte transplantation (XHT) and the rejection mechanisms. Methods The rats with hepatic failure induced by D GI were divided into 2 groups: CsA treated (10 mg/kg per day by intraperitoneal injection) group and CsA untreated group. The hepatocytes of guinea pig were transplanted into the spleen of the rats. The splenic pathohistological examination in the recipients was performed and the 2 week survival rate in the 2 groups observed. Sandwich ELISA method was used to determine the serum levels of interleukin 2 in the rats. Results The survival rate in the CsA treated group ( 78.6 % ) and CsA untreated group (80.0 %) had no significant difference ( P >0.05). But CsA could attenuate the infiltration of inflammatory cells. No significant difference was found in the levels of serum IL 2 of the rats among the normal (28.7±7.0) ng/L, CsA treated(28.5±6.0) ng/L and CsA untreated groups [(31.5±8.0) ng/L, P >0.05]. Conclusion Cellular immune response would occur later and would be weaker in xenogenic hepatocellular transplantation. CsA has no significant therapeutic effects on XHT. [

关 键 词：肝细胞移植 异种移植 环孢素A 排斥反应 

分 类 号：R657.3[医药卫生—外科学] R392.4[医药卫生—临床医学]