猪带绦虫囊尾蚴囊液谷胱甘肽S转换酶活性测定  

Detection of glutathione S-transferase in Cysticercus cellulosae

作　　者：孙新[1] 胡守锋[1] 陈兴智[1] 夏惠[1] 方强[1] 陈兴保[1] 

机构地区：[1]蚌埠医学院寄生虫学教研室,安徽蚌埠233003

出　　处：《蚌埠医学院学报》2001年第1期1-2,共2页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助项目!(96JL0089)

摘　　要：目的：探讨猪带绦虫囊尾蚴囊液内谷胱甘肽S转换酶(GST)的活性。方法：测定酶催化反应后底物浓度的变化，计算GST活力单位。结果：囊液经1∶20稀释，重复4次测定酶促管与非酶促管吸光值，测得平均GST活性为61.84活力单位；囊液冻干品则几无GST活性。结论：囊液中存在游离形式的GST活性，对筛选保护性抗原和可能的化疗靶分子具有潜在意义。Objective:To detect the glutathione S－trans ferase(GST) activity in the fluid of the larvae of Taenia solium(Cysticercus c ellulosae).Methods:By detecting the concentration of the substrate after enzyme added the activity of the enzyme in the fluid was calculated. Results:The GST activity was about 61.84 unit of activity in the flu id after diluted 1∶20 with H\-2O. Conclusions:The results demonstrated that there is GST activity in t he cystic fluid,which shows potential significance in screening protective antig en and possible molecule target for chemotherapy.

关 键 词：囊尾蚴 谷胱甘肽转S换酶类 活化 GST 囊液 

分 类 号：R383.34[医药卫生—医学寄生虫学] Q555[医药卫生—基础医学]