多囊卵巢综合征早期自然流产患者IGFBP-1水平测定及临床意义  

Expression and clinical significance of IGFBP-1 in early spontaneous abortion patients with polycystic ovary syndrome

作　　者：郝明[1] 周楠[1] 王庆琴[1] 

机构地区：[1]中国人民解放军白求恩国际和平医院妇产科,石家庄市050082

出　　处：《河北医药》2013年第23期3525-3527,共3页Hebei Medical Journal

基　　金：河北省人口和计划生育委员会科技研究计划项目(编号:2012-A13)

摘　　要：目的探讨胰岛素样生长因子结合蛋白1（IGFBP-1）在多囊卵巢综合征（polycysticovarysyn—drome，PCOS）早期自然流产患者中的表达及临床意义。方法选择妊娠〈12周的PCOS流产患者77例为试验组，同期正常妊娠〈12周要求人工流产者68例为对照组。2组均于孕3～5周、孕6～8周和孕9～11周采用酶联免疫吸附法（enzyme—linkedimmunosorbentassay，ELISA）检测早期外周静脉血中IGFBP-1、IGF-1、人性激素结合球蛋白（SHBG）、空腹胰岛素、空腹血糖、睾酮和游离睾酮，同时采用流式细胞术检测蜕膜组织中IGFBP-1和IGF-1的表达。结果试验纽孕3～5周和孕9—11周空腹血清胰岛素水平及孕3～5周空腹血糖显著高于对照组（P〈0．01），但孕6—8周血清胰岛素水平及孕6—8周和孕9～11周空腹血糖与对照组比较差异无统计学意义（P〉0．05）。不同孕周试验组睾酮和游离睾酮均显著高于对照组（P〈0．01），IGFBP．1和SHBG水平显著低于对照组（P〈0．05或〈0．01），IGF．1水平与对照组比较差异无统计学意义（P〉0．05）。以B—actin作为内参照，试验组不同孕周蜕膜组织中IGFBP—l蛋白的表达显著低于对照组（P〈0．05），而IGF-1蛋白的表达显著高于对照组（P〈0．05）。结论PCOS早期自然流产患者血清中IGFBP-1、SHBG水平降低以及流产蜕膜组织中IGFBP-1、IGF-1蛋白表达的减少，提示子宫内膜上皮及间质功能障碍可能是PCOS患者发生流产的重要环节。Objective To investigate the expression and clinical significance of IGFBP-1 in early spontaneous abortion patients with polycystic ovary syndrome. Methods 77 early spontaneous abortion patients with PCOS （pregnant period 〈 12 weeks） were enrolled as experimental group, and 68 normal pregnant women （pregnant period 〈 12 weeks ） who asked for induced abortion were enrolled as control group. The levels of IGFBP-1 ,IGF-1 ,SHBG, fasting insulin, fasting glucose, total testosterone and free testosterone in peripheral vein blood were detected by ELISA respectively in 3 N 5 weeks, 6 ~ 8 weeks, 9 ~ 11 weeks after pregnancy for both groups. The expression levels of IGFBP-1 and IGF-1 in decidua tissue were detected by flow cytometry. Results As compared with those in control group, the levels of fasting insulin in 3 - 5wk and 9 -11wk and fasting glucose in 3 - 5wk were significantly increased in experimental group （ P 〈 0.01 ）, however, there were no significant differences in the levels of fasting insulin in 6 - 8wk and fasting glucose in 6 - 8wk and 9 - llwk between experimental group and control group （ P 〉 O. 05 ）. The levels of testosterone and free testosterone in the three experimental groups were significantly higher than those in control group （ P 〈 0. O1 ）, however, the levels of IGFBP-land SHBG were significantly lower than those in control group （ P 〈 O. 05 or 〈 0.01 ）. There were no significant differences in the levels of IGF-1 between experimental group and control group （ P 〉 O. 05 ）. Taking 13-actin as interior reference, the expression levels of IGFBP-1 in deeidua tissue in experimental group were significantly lower than those in control group （ P 〈 O. 05）, however, the expression levels of IGF-I in decidua tissue in experimental group were significantly higher than those in control group （ P 〈 0.05 ）. Conclusion The reduction of serum IGFBP-1 and SHBG concentrations and decrease of expression levels of IGFBP-1 and IGF-1 in decidua tissue of early s

关 键 词：胰岛素样生长因子结合蛋白1 多囊卵巢综合征 酶联免疫吸附法 

分 类 号：R714.21[医药卫生—妇产科学]