细胞色素c在4-氨基-6-羟基-2-巯基嘧啶修饰金电极上的直接电化学行为  

Direct Electrochemical Behaviors of Cytochrome c on 4-Amino-6-Hydroxy-2-Mercaptopyrimidine Modified Gold Electrode

作　　者：白林山[1] 吕丹丹[1] 周影[1] 

机构地区：[1]安徽工业大学化学与化工学院,安徽马鞍山243002

出　　处：《安徽工业大学学报（自然科学版）》2013年第4期403-408,共6页Journal of Anhui University of Technology（Natural Science）

基　　金：安徽工业大学研究生创新研究基金项目(2010009)

摘　　要：用循环伏安法研究细胞色素c在4-氨基-6-羟基-2-巯基嘧啶(AHMP)自组装修饰金电极上的直接电化学行为。对修饰条件进行优化,通过扫描电镜(SEM)等方法对修饰电极进行了表征。结果表明,细胞色素c在AHMP修饰金电极上能进行准可逆的电化学反应,氧化还原峰电位差(ΔEp)为67 mV。氧化与还原峰电流比ipa/ipc接近1,且峰电流与电位扫描速率的平方根(v1/2)呈正比,属于扩散控制的准可逆过程,异相电子迁移速率常数(Ks)为1.93×10-4cm/s。The direct electrochemical behaviors of cytochrome c on 4-amino-6-hydroxy-2-mercaptopyrimidine （AHMP） modified gold electrode were studied with cyclic voltammetry.The modification conditions were optimized,and the AHMP modified gold electrode was characterized by scanning electron microscopy （SEM）.The results show that cytochrome c can carry out quasi-reversible electrochemical reaction on the 4-amino-6-hydroxy-2-mercaptopyrimidine modified gold electrode.The redox potential difference （ΔEp） is 67 mV,and the redox peak current ratio ipa/ipc was nearly 1.The peak current is positively correlated to the square root of potential scanning rate （v1/2）,indicating that it is a diffusion-controlled quasi-reversible process,and the heterogeneous electronmigration rate constant （Ks） is1.93×10-4cm/s.

关 键 词：细胞色素C 4-氨基-6-羟基-2-巯基嘧啶 直接电化学 循环伏安法 化学修饰电极 

分 类 号：O657.1[理学—分析化学]