大理白族2型糖尿病与脂联素基因多态性相关性分析  

Genetic association analysis of the adiponectin polymorphisms in type 2 diabetes of Bai nationality at Dali area

作　　者：康庄[1] 苏恒[1] 张云[1] 虞艳芳[1] 欧杨[1] 薛元明[1] 赵惠珠 

机构地区：[1]云南省第一人民医院内分泌科,昆明650032 [2]云南省大理州人民医院,671000

出　　处：《检验医学与临床》2013年第23期3122-3124,共3页Laboratory Medicine and Clinic

基　　金：云南省卫生厅内设机构科研资助项目(2009NS015);中华医学会临床医学科研专项资金(10020130235)

摘　　要：目的检测云南省大理白族脂联素基因启动子单核苷酸多态性(SNP)与2型糖尿病的相关性。方法采用聚合酶链反应直接测序法在300例无血缘关系的云南省大理白族人群(健康者120例,2型糖尿病患者180例)中检测SNPs+45、+276的基因型,分析以上2个多态性位点与2型糖尿病的相关性。结果 SNP+45位点在健康者和2型糖尿病患者具有明显差异,SNP+276在两组差异无统计学意义。连锁不平衡分析显示,健康组和病例组间SNPs+45、+276存在一定差异。结论大理白族人群脂联素基因上SNP+45G与2型糖尿病的发生存在关联;SNP+45G是2型糖尿病发生的一项危险因素。Objective Adiponectin gene is associated with BMI,insulin sensitivity and diabetes. To detect the impact of adiponectin gene promoter single nucleotide polymorphism（SNP） on type Ⅱ diabetes in Bai nationality of Yunnan province. Methods We detected adiponectin gene ＋276G/T and ＋45T/G with PCR- direct sequencing in 300 cases of unrelated people which contain healthy control subjects（n= 120） and type 2 diabetic patients（n= 180） in Bai nationality of Yunnan province, then analyzed their relationship with type 2 diabetes. Results SNP＋ 45 （G/T） showed significant difference of allele frequencies between type 2 diabetic group and the control group（P = 0.013）. Not statistically significant difference was found at adiponectin gene polymorphisms ＋276G/T in this study. Linkage disequilibrium（LD） of ＋ 276G/T and ＋ 45T/G between cases and controls showed significant differences. Conclusion The present study provides evidence that SNP＋45G in exon 2 region of the adiponectin gene contributes to the de- velopment of type 2 diabetes in Bai nationality of Yunnan province.

关 键 词：脂联素基因 单核苷酸多态性 2型糖尿病 大理白族 

分 类 号：R587.1[医药卫生—内分泌]